De Vrind H H, Wondergem J, Haveman J
Department of Radiotherapy, University of Amsterdam, The Netherlands.
J Neurosci Methods. 1992 Dec;45(3):165-74. doi: 10.1016/0165-0270(92)90073-m.
A 5-mm segment of the rat sciatic nerve was treated in vivo with hyperthermia (43-45 degrees C) for different times using a brass thermode. The effect of this local heat treatment on the nerve was assessed with electrophysiology and using two functional assays. Hyperthermia led to a dose-dependent decrease of motor and sensory function. Electrophysiological examination showed a decrease in amplitude of motor and reflex responses rather than a decrease in conduction velocities. Calculated ED50 values were not significantly different for the two functional and for the electrophysiological methods. Functional recovery from nerve damage took place in all cases. Measured at the same level of damage, i.e., 50% function loss, it took 14 days to recover from complete sensory function loss and 20 days from complete motor function loss. Although both motor and sensory functions were restored, 30 days after hyperthermia no responses could be detected with electrophysiology, this as a result of the thin myelin sheaths that occur upon recovery.
使用黄铜热电极对大鼠坐骨神经的5毫米节段进行体内高温（43 - 45摄氏度）处理不同时间。通过电生理学和两种功能测定法评估这种局部热处理对神经的影响。高温导致运动和感觉功能呈剂量依赖性下降。电生理检查显示运动和反射反应的幅度降低，而非传导速度下降。两种功能测定法和电生理方法计算出的半数有效剂量（ED50）值无显著差异。所有病例中神经损伤均出现功能恢复。在相同损伤水平（即功能丧失50%）下测量，完全感觉功能丧失后恢复需14天，完全运动功能丧失后恢复需20天。尽管运动和感觉功能均得以恢复，但高温处理30天后，电生理学检测未发现反应，这是恢复时出现薄髓鞘的结果。
