Uhl E, Sirsjö A, Haapaniemi T, Nilsson G, Nylander G
Institute for Surgical Research, Ludwig-Maximilians University, Munich, Germany.
Plast Reconstr Surg. 1994 Apr;93(4):835-41. doi: 10.1097/00006534-199404000-00028.
The influence of hyperbaric oxygen on reepithelialization and on microvascular perfusion of wounds in normal and ischemic skin tissue was investigated by using a standardized model, in ears of hairless mice. Animals were treated within 2 hours of wound creation and then twice daily with 100% oxygen at 2 atmospheres of absolute pressure. Ischemia was induced by ligating two of the three major vessels of the ear 2.5 days before wound creation. Wound surface area was measured every third day after wound creation. In addition, microvascular blood flow before and during the wound healing process was measured by scanning the ear with a new laser Doppler perfusion imager. In normal tissue (n = 13), hyperbaric oxygen therapy significantly accelerated wound healing by 2 days (p < 0.01) as compared with controls (n = 16). In ischemic tissue (n = 16), treatment with hyperbaric oxygen reduced time for reepithelialization in control animals (n = 16) from 14.3 to 9.9 days (p < 0.001). Laser Doppler data showed no difference in tissue blood flow between treated and untreated animals. In comparison with normal tissue, wound healing in ischemic tissue was characterized by a reduced and less intense hyperemic response. These data suggest that hyperbaric oxygen therapy improves reepithelialization in normal and ischemic skin tissue. The beneficial effect is not associated with changes in microvascular perfusion and therefore is probably due to high arteriolar oxygen content and oxygen diffusion.
通过使用标准化模型，在无毛小鼠耳部研究了高压氧对正常和缺血性皮肤组织伤口再上皮化及微血管灌注的影响。在伤口形成后2小时内对动物进行治疗，然后每天两次在绝对压力2个大气压下给予100%氧气。在伤口形成前2.5天，通过结扎耳部三条主要血管中的两条来诱导缺血。伤口形成后每隔一天测量伤口表面积。此外，在伤口愈合过程之前和期间，通过使用新型激光多普勒灌注成像仪扫描耳部来测量微血管血流。在正常组织（n = 13）中，与对照组（n = 16）相比，高压氧治疗显著加速伤口愈合2天（p < 0.01）。在缺血组织（n = 16）中，高压氧治疗使对照动物（n = 16）的再上皮化时间从14.3天缩短至9.9天（p < 0.001）。激光多普勒数据显示，治疗组和未治疗组动物的组织血流无差异。与正常组织相比，缺血组织中的伤口愈合表现为充血反应减弱且强度降低。这些数据表明，高压氧治疗可改善正常和缺血性皮肤组织的再上皮化。这种有益作用与微血管灌注的变化无关，因此可能是由于小动脉中高氧含量和氧扩散所致。
