Ker C G, Wu S C
Department of Surgery, Kaohsiung Medical College, Taiwan, Republic of China.
Gaoxiong Yi Xue Ke Xue Za Zhi. 1992 Oct;8(10):520-4.
Surgical jaundice can be easily induced by ligation of bile duct, but it is not easy to reverse the ligation in rats. Therefore, a simple method was designed in our laboratory to address the problem. Obstruction of bile duct can be achieved by the compression by two arms of a fine silicon tube enveloped in an outer bigger silicon tube. Both silicon tubes are then fixed and sutured to the abdominal peritoneum. Therefore, if the releasing of the obstruction is required, removal of the inner fine silicon tube by removing off the previous suture tire of silicon tube which had settled under the skin. Thus, the reversing of obstruction could be achieved without extensive exploration into the abdominal cavity. One week after obstruction the bile duct of rats were dialated 6.10 +/- 1.26 mm (Mean +/- SD) in rats. The serum bilirubin and alkaline phosphatase were 6.29 +/- 1.26 mg% and 229.30 +/- 82.22 IU respectively. The dilated bile duct decreased into 3.00 +/- 0.98 mm one week after decompress with this method. Serum bilirubin and alkaline phosphatase were 1.80 +/- 0.79 mg% and 90.50 +/- 19.60 IU respectively. It proved that this animal model was simple and had the benefit of being able to sample the bile without contamination with intestinal contents after decompression.
通过结扎胆管可轻易诱导出外科性黄疸，但在大鼠中逆转结扎并不容易。因此，我们实验室设计了一种简单方法来解决这个问题。胆管梗阻可通过将一根细硅管的两端包裹在一根更大的外部硅管中进行压迫来实现。然后将两根硅管固定并缝合到腹部腹膜上。因此，如果需要解除梗阻，通过拆除先前埋置于皮下的硅管缝线环来移除内部细硅管即可。这样，无需对腹腔进行广泛探查就能实现梗阻的逆转。梗阻一周后，大鼠胆管扩张至6.10±1.26毫米（均值±标准差）。血清胆红素和碱性磷酸酶分别为6.29±1.26毫克%和229.30±82.22国际单位。用此方法减压一周后，扩张的胆管缩小至3.00±0.98毫米。血清胆红素和碱性磷酸酶分别为1.80±0.79毫克%和90.50±19.60国际单位。这证明该动物模型简单，且具有减压后能采集胆汁而不被肠内容物污染的优点。
