Staack Andrea, Donjacour Annemarie A, Brody Joel, Cunha Gerald R, Carroll Peter
Department of Anatomy, HSW-1323, University of California, 513 Parnassus Avenue, San Francisco, CA 94143, USA.
Differentiation. 2003 Sep;71(7):402-13. doi: 10.1046/j.1432-0436.2003.7107004.x.
A detailed knowledge of the developmental anatomy of the embryonic mouse urogenital tract is required to recognize mutant urogenital phenotypes in transgenic and knock-out mice. Accordingly, the purpose of this article is to review urogenital development in the mouse embryo and to give an illustrated methodological protocol for the dissection of urogenital organ rudiments at 12-13 days of gestation (E12-13) to isolate the urogenital ridge and at E16 to isolate the seminal vesicle, Müllerian duct, Wolffian duct, and prostatic rudiment, the urogenital sinus (UGS). The UGS can be cultured and, in the presence of testosterone, prostatic buds form in vitro. Because of the importance of mesenchymal-epithelial interactions in urogenital development, methods for the isolation of epithelium and mesenchyme from the embryonic urogenital sinus are also described. Urogenital sinus mesenchyme (UGM) and urogenital sinus epithelium (UGE) can be used to construct tissue recombinants that can either be grown in vitro or grafted in vivo for the study of epithelial-mesenchymal interactions in prostatic development.
要识别转基因和基因敲除小鼠的泌尿生殖系统突变表型，需要详细了解胚胎期小鼠泌尿生殖系统的发育解剖学知识。因此，本文的目的是回顾小鼠胚胎期泌尿生殖系统的发育，并给出一个有插图的方法方案，用于在妊娠12 - 13天（E12 - 13）解剖泌尿生殖器官原基以分离泌尿生殖嵴，以及在E16时分离精囊、苗勒管、沃尔夫管、前列腺原基和泌尿生殖窦（UGS）。UGS可以进行培养，并且在睾酮存在的情况下，前列腺芽在体外形成。由于间充质 - 上皮相互作用在泌尿生殖系统发育中的重要性，本文还描述了从胚胎泌尿生殖窦中分离上皮和间充质的方法。泌尿生殖窦间充质（UGM）和泌尿生殖窦上皮（UGE）可用于构建组织重组体，这些重组体既可以在体外培养，也可以在体内移植，用于研究前列腺发育过程中的上皮 - 间充质相互作用。
