Newton S E, Meeusen E N T
Victorian Institute of Animal Science, 475 Mickleham Road, Attwood, Victoria, 3049, Australia.
Parasite Immunol. 2003 May;25(5):283-96. doi: 10.1046/j.1365-3024.2003.00631.x.
Despite the identification of highly effective native antigens for vaccination against Haemonchus contortus, particularly 'hidden' antigens derived from the intestine of adult worms, to date similar efficacy has not been shown with recombinant antigens. In addition, progress towards identification of protective antigens from other sheep gastrointestinal (GI) nematode species is limited. Coupled with this is an incomplete understanding of the mechanism of natural immunity to GI nematodes, making selection of appropriate immunization strategies and adjuvants for evaluation of candidate 'natural' antigens problematic. The current explosion in new high-throughput technologies, arising from human studies, for analysis of the genome, transcriptome, proteome and glycome offers the opportunity to gain a better understanding of the molecular pathways underlying pathogen biology, the host immune system and the host-pathogen interaction. An overview is provided on how these technologies can be applied to parasite research and how they may aid in overcoming some of the current problems in development of commercial vaccines against GI nematode parasites.
尽管已鉴定出针对捻转血矛线虫进行疫苗接种的高效天然抗原，特别是源自成虫肠道的“隐蔽”抗原，但迄今为止，重组抗原尚未显示出类似的效果。此外，从其他绵羊胃肠道线虫物种中鉴定保护性抗原的进展有限。与此同时，对胃肠道线虫自然免疫机制的理解并不完整，这使得选择合适的免疫策略和佐剂来评估候选“天然”抗原变得困难。当前源于人类研究的用于分析基因组、转录组、蛋白质组和糖组的新型高通量技术的迅猛发展，为更好地理解病原体生物学、宿主免疫系统以及宿主 - 病原体相互作用的分子途径提供了机会。本文概述了这些技术如何应用于寄生虫研究，以及它们如何有助于克服目前在开发针对胃肠道线虫寄生虫的商业疫苗方面的一些问题。
