Chang Cheng Fang, Su Mao Sen, Chen Houng Yung, Liao I Chiu
Tungkang Marine Laboratory, Taiwan Fisheries Research Institute, Tungkang, Pingtung 928, Taiwan, ROC.
Fish Shellfish Immunol. 2003 Oct;15(4):297-310. doi: 10.1016/s1050-4648(02)00167-5.
The effectiveness of dietary beta-1,3-glucan (BG), derived from Schizophyllum commune, in modulating the non-specific immunity of the grass prawn Penaeus monodon and its resistance to white spot syndrome virus (WSSV) were investigated. Juvenile P. monodon (6.5+/-0.4 g) were fed for 20 days on a series of test diets containing graded levels of BG (0, 1, 2, 10, 20 g kg(-1)diet) and were then challenged by injection of WSSV. The haemolymph total haemocyte count (THC), phagocytosis (PI), phenoloxidase (PO), superoxide anion (O(2)(-)) and superoxide dismutase (SOD) production were measured at days 0, 1, 3, 6, 9, 12 and 24 after challenge, and shrimp survival rate was also recorded. All the shrimps fed on diets containing BG no more than 1 g kg(-1)died by day 12. Conversely, the survival rate of shrimp fed with the diet containing 10 g kg(-1)BG was significantly higher (P<0.05) by day 9 than that of the other groups. When screened by the WSSV PCR diagnostic procedure, the percentages of surviving juveniles of the BG 2, 10, 20 g kg(-1)groups that were 2-step WSSV negative, were 55, 65 and 65%, respectively. The haemolymph THC, PO, O(2)(-)and SOD production of the 2, 10 and 20 g kg(-1)BG diet groups dropped drastically immediately after the WSSV challenge but subsequently returned to normal. Therefore, oral administration of BG at an optimal level of 10 g kg(-1)diet for 20 days effectively enhanced the immune system and improved the survival of WSSV-infected P. monodon.
研究了源自裂褶菌的膳食β-1,3-葡聚糖（BG）对斑节对虾非特异性免疫的调节作用及其对白斑综合征病毒（WSSV）的抗性。将斑节对虾幼体（6.5±0.4克）投喂含不同梯度水平BG（0、1、2、10、20克/千克饲料）的一系列试验饲料20天，然后通过注射WSSV进行攻毒。在攻毒后第0、1、3、6、9、12和24天测定血淋巴总血细胞计数（THC）、吞噬作用（PI）、酚氧化酶（PO）、超氧阴离子（O₂⁻）和超氧化物歧化酶（SOD）活性，并记录对虾存活率。所有投喂BG含量不超过1克/千克饲料的对虾在第12天全部死亡。相反，投喂含10克/千克BG饲料的对虾在第9天的存活率显著高于其他组（P<0.05）。采用WSSV PCR诊断程序检测时，BG 2、10、20克/千克组存活幼体中两步WSSV阴性的百分比分别为55%、65%和65%。2、10和20克/千克BG饲料组的血淋巴THC、PO、O₂⁻和SOD活性在WSSV攻毒后立即急剧下降，但随后恢复正常。因此，以10克/千克饲料的最佳水平口服BG 20天可有效增强免疫系统，提高感染WSSV的斑节对虾的存活率。
