Lu Zhong-fa, Wu Jin-jin, Liu Rong-qing, Zhong Bai-yu, Zheng Min
The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2003 Aug;32(4):323-6. doi: 10.3785/j.issn.1008-9292.2003.04.013.
To investigate the effects of rat tail collagen, hair follicle dermal papilla cells and hair follicle epithelium cells on collagen gel contraction in organotypic culture.
The hair follicle organotypic culture was prepared with different concentrations of rat tail collagen, different number of dermal papilla cells and hair follicle epithelium cells in DMEM medium, after cultured for 10 days the diameter of collagen gel was measured.
The concentration of rat tail collagen, hair follicle dermal papilla cells and hair follicle epithelium cells significantly influenced on collagen gel contraction in organotypic culture (P<0.01). The contraction of collagen gel was negatively related to the concentration of rat tail collagen, while the concentration of dermal papilla cells and hair follicle epithelium cells was positively related to the contraction of collagen gel.
The key factor influencing collagen gel contraction in organotypic culture is the concentration of rat tail collagen, hair follicle dermal papilla cells and hair follicle epithelium cells.
研究大鼠尾胶原、毛囊真皮乳头细胞和毛囊上皮细胞在器官型培养中对胶原凝胶收缩的影响。
在DMEM培养基中用不同浓度的大鼠尾胶原、不同数量的真皮乳头细胞和毛囊上皮细胞制备毛囊器官型培养物，培养10天后测量胶原凝胶的直径。
大鼠尾胶原、毛囊真皮乳头细胞和毛囊上皮细胞的浓度对器官型培养中胶原凝胶的收缩有显著影响（P<0.01）。胶原凝胶的收缩与大鼠尾胶原的浓度呈负相关，而真皮乳头细胞和毛囊上皮细胞的浓度与胶原凝胶的收缩呈正相关。
影响器官型培养中胶原凝胶收缩的关键因素是大鼠尾胶原、毛囊真皮乳头细胞和毛囊上皮细胞的浓度。
