Beikler Thomas, Peters Ulrike, Prajaneh Seangsome, Prior Karola, Ehmke Benjamin, Flemmig Thomas Frank
Department of Periodontology, University of Münster, Münster, Germany.
Eur J Oral Sci. 2003 Oct;111(5):390-4. doi: 10.1034/j.1600-0722.2003.00065.x.
The aim of the present study was to determine the prevalence of Porphyromonas gingivalis fimA genotypes in Caucasian patients with periodontitis. A total of 102 patients harboring P. gingivalis subgingivally were enrolled into the study. Pooled subgingival plaque samples of the six most severely affected sites were taken and analysed by fimA-specific polymerase chain reaction (PCR) and restriction analysis. Moreover, 26 P. gingivalis isolates were analysed by sequence analysis of the fimA gene. Sequence analysis revealed five major fimA genotypes (fimA types I-V) and allowed further subtyping of fimA genotypes II and IV into two subgroups each. The overall prevalences of fimA genotypes as assessed by PCR and restriction analysis among the P. gingivalis-positive patients with periodontitis were: type I, 25.5%; type II, 38.2%; type III, 4.9%; type IV, 18.6%; type V, 3.9%; and non-typable, 6.9%. Two patients were colonized by both type II and type IV, or type III and type IV fimA genotypes, respectively. Patients harboring different fimA genotypes showed no significant difference in severity of periodontal disease, as assessed by pocket probing depth and bleeding on probing following adjustment for smoking habit and age. The results indicate that predominant fimA genotypes in Caucasian periodontitis patients are types I, II, and IV. However, there was no difference in the association of the various fimA genotypes with disease severity.
本研究的目的是确定白种人牙周炎患者牙龈卟啉单胞菌fimA基因型的流行情况。共有102例龈下存在牙龈卟啉单胞菌的患者纳入本研究。采集六个受影响最严重部位的龈下菌斑混合样本，通过fimA特异性聚合酶链反应（PCR）和限制性分析进行检测。此外，对26株牙龈卟啉单胞菌分离株进行fimA基因序列分析。序列分析揭示了五种主要的fimA基因型（fimA I型至V型），并允许将fimA II型和IV型基因型进一步细分为两个亚组。通过PCR和限制性分析评估，牙周炎牙龈卟啉单胞菌阳性患者中fimA基因型的总体流行率为：I型，25.5%；II型，38.2%；III型，4.9%；IV型，18.6%；V型，3.9%；不可分型，6.9%。两名患者分别被II型和IV型或III型和IV型fimA基因型定植。在根据吸烟习惯和年龄进行调整后，通过牙周袋探诊深度和探诊出血评估，携带不同fimA基因型的患者在牙周疾病严重程度上没有显著差异。结果表明，白种人牙周炎患者中主要的fimA基因型为I型、II型和IV型。然而，各种fimA基因型与疾病严重程度之间的关联没有差异。
