Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences & Research Centre, Bauxite Road, Belagavi, Karnataka 590010, India.
B.V. Bhoomaraddi College of Engineering and Technology, KLE Technological University, Vidya Nagar, Hubballi, Karnataka 580031, India.
Int J Environ Res Public Health. 2020 Mar 11;17(6):1826. doi: 10.3390/ijerph17061826.
is regarded as a "keystone pathogen" in periodontitis. The fimbria assists in the initial attachment, biofilm organization, and bacterial adhesion leading to the invasion and colonization of host epithelial cells. The present study aimed to investigate the occurrence of genotypes in patients with chronic periodontitis and healthy individuals in the Indian population, and to study their association with the number of cells obtained in subgingival plaque samples of these subjects. The study comprised 95 samples from the chronic periodontitis (CP) group and 35 samples from the healthy (H) group, which were detected positive for in our previous study. Fimbrial genotyping was done by PCR and PCR-restriction fragment length polymorphism (RFLP). The type II was more prevalent in the CP group (55.89%), followed by type IV (30.52%), whereas in the H group, type I was the most prevalent fimbria (51.42%). The quantity of cells increased with the presence of types II and III. Our results suggest a strong relationship between types II and IV and periodontitis, and between type I and the healthy condition. The colonization of organisms was increased with the occurrence of type II in deep periodontal sites, which could play an important role in the progression of the disease.
被认为是牙周炎的“关键病原体”。菌毛有助于初始附着、生物膜组织和细菌黏附，从而导致宿主上皮细胞的入侵和定植。本研究旨在调查基因型在印度人群慢性牙周炎患者和健康个体中的发生情况，并研究其与这些受试者龈下菌斑样本中获得的 细胞数量的关系。该研究包括来自慢性牙周炎（CP）组的 95 个样本和来自健康（H）组的 35 个样本，这些样本在我们之前的研究中被检测为阳性。菌毛基因分型通过 PCR 和 PCR-限制性片段长度多态性（RFLP）进行。在 CP 组中，II 型更为常见（55.89%），其次是 IV 型（30.52%），而在 H 组中，I 型是最常见的菌毛（51.42%）。随着 II 型和 III 型的存在， 细胞的数量增加。我们的结果表明，II 型和 IV 型与牙周炎之间以及 I 型与健康状况之间存在很强的关系。在深牙周部位，II 型的发生增加了生物体的定植，这可能在疾病的进展中起重要作用。
