Cardosa Mary Jane, Wang Seok Mui, Sum Magdline Sia H, Tio Phaik Hooi
Institute of Health & Community Medicine, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
BMC Microbiol. 2002 May 5;2:9. doi: 10.1186/1471-2180-2-9.
In Southeast Asia, dengue viruses often co-circulate with other flaviviruses such as Japanese encephalitis virus, and due to the presence of shared antigenic epitopes it is often difficult to use serological methods to distinguish between previous infections by these flaviviruses.
Convalescent sera from 69 individuals who were known to have had dengue or Japanese encephalitis virus infection were tested by western blotting against dengue, Japanese encephalitis and West Nile virus antigens. We determined that individuals who had been infected with dengue viruses had IgG responses against the premembrane protein of dengue viruses but not Japanese encephalitis, whereas individuals who had been infected with Japanese encephalitis had IgG specific for the premembrane protein of Japanese encephalitis virus but not the dengue viruses. None reacted with the premembrane protein of West Nile virus. Using the Pearson Chi Square test, it was determined that the difference between the two groups was highly significant with a p value of <0.001.
The use of flavivirus premembrane protein in seroepidemiological studies will be useful in determining what flaviviruses have circulated in a community.
在东南亚，登革病毒常常与其他黄病毒共同传播，例如日本脑炎病毒，并且由于存在共同的抗原表位，使用血清学方法区分这些黄病毒先前的感染往往很困难。
对已知感染过登革热或日本脑炎病毒的69名个体的恢复期血清进行了蛋白质印迹法检测，检测针对登革热、日本脑炎和西尼罗河病毒抗原的抗体。我们确定，感染过登革病毒的个体对登革病毒的前膜蛋白有IgG反应，但对日本脑炎病毒没有反应，而感染过日本脑炎的个体对日本脑炎病毒的前膜蛋白有IgG特异性反应，但对登革病毒没有反应。没有人对西尼罗河病毒的前膜蛋白产生反应。使用Pearson卡方检验，确定两组之间的差异非常显著，p值<0.001。
在血清流行病学研究中使用黄病毒前膜蛋白将有助于确定在一个社区中传播过哪些黄病毒。
