Mayevsky A, Nioka S, Chance B
Life Sciences Dept., Bar-Ilan University, Ramat-Gan, Israel.
Adv Exp Med Biol. 1988;222:365-74. doi: 10.1007/978-1-4615-9510-6_43.
Various members of the respiratory chain exhibit different optical properties in the reduced and oxidized forms, thus enabling the non-invasive monitoring of various organs in vitro as well as in vivo. Since the pioneering work of Chance, Cohen, Jobsis and Schoener in 1962, many groups of investigators adopted their approach in monitoring NADH oxidation reduction states in vivo for the brain as well as for other body organs. In 1972, we introduced flexible, optical fibers into the surface fluorometry replacing the usual "rigid" optical system used by other groups. During the last decade, this technique has been developed, improved and applied to many experimental setups in brain research and very recently was combined with 31P NMR spectroscopy for the puppy and the adult dog brain in vivo. In our system, the effects of movement artifacts and changes in blood oxygenation are negligible while the effects of tissue absorption or blood volume changes are considerable and could be minimized by subtraction of the reflectance signal from that of the fluorescence (1:1 ratio) providing the corrected fluorescence signal.
呼吸链的不同成员在还原态和氧化态下表现出不同的光学特性，从而能够在体外和体内对各种器官进行非侵入性监测。自1962年钱斯、科恩、乔布西斯和舍纳的开创性工作以来，许多研究小组采用他们的方法在体内监测大脑以及其他身体器官中NADH的氧化还原状态。1972年，我们将柔性光纤引入表面荧光测定法，取代了其他小组常用的“刚性”光学系统。在过去十年中，这项技术得到了发展、改进，并应用于许多脑研究的实验装置中，最近还与幼犬和成年犬大脑的体内31P核磁共振波谱相结合。在我们的系统中，运动伪影和血液氧合变化的影响可以忽略不计，而组织吸收或血容量变化的影响相当大，可以通过从荧光信号（1:1比例）中减去反射信号来最小化，从而提供校正后的荧光信号。
