Cohen G H, Ponce de Leon M, Diggelmann H, Lawrence W C, Vernon S K, Eisenberg R J
J Virol. 1980 May;34(2):521-31. doi: 10.1128/JVI.34.2.521-531.1980.
Capsids of herpes simplex virus (HSV) types 1 and 2 contain seven polypeptides ranging in molecular weight from 154,000 to 12,000 (termed NC-1 through NC-7 in order of descending molecular weight). Antibodies prepared to HSV-1 capsid polypeptides isolated from sodium dodecyl sulfate-polyacrylamide gels reacted in an immunofluorescence assay against HSV-1-infected KB cells. Three of the antibodies (anti-NC-1, anti-NC-2, and anti-NC-3,4) also reacted with HSV-2-infected cells. Tryptic peptide analysis showed that each of the HSV-1 capsid polypeptides had a unique methionine peptide profile, and none appeared to be derived from the major capsid polypeptide. Comparative peptide analysis of HSV-1 and HSV-2 showed that one polypeptide (NC-7, 12,000 molecular weight) had an identical methionine peptide profile and a very similar arginine peptide profile in both virus types. The arginine peptide profile of NC-7 of HSV-1 was very different from the arginine profile of KB histone H4. Although there were certain intertypic similarities in the methionine peptide profiles of the other capsid components especially in NC-1 (the major capsid protein), there was no case where the tryptic peptides were identical in the two virus types.
1型和2型单纯疱疹病毒（HSV）的衣壳包含7种多肽，分子量从154,000到12,000不等（按照分子量降序依次称为NC-1至NC-7）。针对从十二烷基硫酸钠-聚丙烯酰胺凝胶中分离出的HSV-1衣壳多肽制备的抗体，在免疫荧光试验中与HSV-1感染的KB细胞发生反应。其中三种抗体（抗-NC-1、抗-NC-2和抗-NC-3,4）也与HSV-2感染的细胞发生反应。胰蛋白酶肽分析表明，HSV-1衣壳的每种多肽都有独特的甲硫氨酸肽谱，且似乎都不是源自主要衣壳多肽。HSV-1和HSV-2的肽比较分析表明，有一种多肽（NC-7，分子量12,000）在两种病毒类型中具有相同的甲硫氨酸肽谱和非常相似的精氨酸肽谱。HSV-1的NC-7的精氨酸肽谱与KB组蛋白H4的精氨酸谱非常不同。尽管其他衣壳成分的甲硫氨酸肽谱存在某些型间相似性，尤其是在NC-1（主要衣壳蛋白）中，但在两种病毒类型中胰蛋白酶肽都不相同。
