Ozaki T, Hayashi H, Tani K, Ogushi F, Yasuoka S, Ogura T
Third Department of Internal Medicine, School of Medicine, Tokushima University, Japan.
Am Rev Respir Dis. 1992 Jan;145(1):85-91. doi: 10.1164/ajrccm/145.1.85.
This study was designed to clarify the contributions of specific neutrophil chemotactic factors (NCF) in neutrophil accumulation in the human respiratory tract associated with various diseases. The activity and characteristics of the NCF in the bronchoalveolar lavage (BAL) fluid and culture media of alveolar macrophages obtained from normal volunteers, control patients, patients with chronic airway diseases (CAD) and patients with idiopathic pulmonary fibrosis (IPF) were examined. The BAL fluid from normal volunteers contained NCF comparable with the chemotactic factors interleukin-8 (IL-8) and leukotriene B4 (LTB4). Analysis of the biochemical characteristics of NCF released from alveolar macrophages suggests that they are derived from alveolar macrophages. The NCF activities in BAL fluids from patients with CAD and IPF were higher than those in BAL fluids from normal volunteers and control patients. Biochemical analysis demonstrated that several kinds of NCF, including those derived from the complement component C5 and alveolar macrophages, were present in the BAL fluid from patients with CAD and respiratory infections. The especially marked increase of C5-derived NCF indicate their importance in neutrophil accumulation in the respiratory tract of patients with CAD. Alveolar macrophages released different types of NCF after different lengths of culture periods (4 h and 24 h). Alveolar macrophages from patients with IPF released larger amounts of NCF than alveolar macrophages from normal volunteers, indicating the importance of alveolar-macrophage-derived NCF as well as C5-derived NCF in neutrophil accumulation in the respiratory tract of patients with IPF. These results suggest that various types of NCF increase in response to different disease states of the respiratory tract and serve to regulate the accumulation of neutrophils.
本研究旨在阐明特定中性粒细胞趋化因子（NCF）在与各种疾病相关的人类呼吸道中性粒细胞积聚中的作用。检测了从正常志愿者、对照患者、慢性气道疾病（CAD）患者和特发性肺纤维化（IPF）患者获取的支气管肺泡灌洗（BAL）液及肺泡巨噬细胞培养基中NCF的活性和特性。正常志愿者的BAL液中所含NCF与趋化因子白细胞介素-8（IL-8）和白三烯B4（LTB4）相当。对肺泡巨噬细胞释放的NCF进行生化特性分析表明，它们源自肺泡巨噬细胞。CAD患者和IPF患者BAL液中的NCF活性高于正常志愿者和对照患者BAL液中的活性。生化分析表明，CAD患者和呼吸道感染患者的BAL液中存在几种NCF，包括源自补体成分C5和肺泡巨噬细胞的NCF。源自C5的NCF显著增加表明它们在CAD患者呼吸道中性粒细胞积聚中具有重要作用。肺泡巨噬细胞在不同培养时间（4小时和24小时）后释放不同类型的NCF。IPF患者的肺泡巨噬细胞比正常志愿者的肺泡巨噬细胞释放更多的NCF，这表明肺泡巨噬细胞源性NCF以及C5源性NCF在IPF患者呼吸道中性粒细胞积聚中具有重要作用。这些结果表明，各种类型的NCF会随着呼吸道不同疾病状态而增加，并有助于调节中性粒细胞的积聚。
