Walboomers J M, Fokke H E, Polak M, Volkers H, Houthoff H J, Barents J, van der Noordaa J, ter Schegget J
Department of Pathology, University of Amsterdam, The Netherlands.
Intervirology. 1987;27(2):81-5. doi: 10.1159/000149723.
Metastatic tissue from an endocervical adenocarcinoma was examined for human papilloma virus (HPV) DNA sequences. Southern blot analysis with 32P-labeled viral probes revealed the presence of about 150 copies of HPV-16 DNA per cellular genome. DNA in situ hybridization of frozen sections with biotinylated probes showed that HPV-16 DNA was exclusively detectable in the nuclei of the malignant epithelial cells and not in the stroma.
对1例宫颈管腺癌的转移组织进行人乳头瘤病毒（HPV）DNA序列检测。用32P标记的病毒探针进行Southern印迹分析显示，每个细胞基因组中存在约150个HPV-16 DNA拷贝。用生物素化探针进行冷冻切片的DNA原位杂交显示，HPV-16 DNA仅在恶性上皮细胞核中可检测到，而在基质中未检测到。
