Iglesias G J, Trujano M, Lokensgard J, Molitor T
Department of Food Animal and Equine Medicine, College of Veterinary Medicine, North Carolina State University, Raleigh 27606.
Can J Vet Res. 1992 Jan;56(1):74-7.
In order to investigate the potential involvement of pseudorabies virus (PRV) in swine respiratory disease, nine week old pigs were intranasally inoculated with the PRV strain 4892. Two doses of infection were used: 10(4.5) median tissue culture infectious doses (TCID50)/pig and 10(3.5) TCID50/pig, with ten pigs per group. In the group of pigs inoculated with 10(4.5) TCID50, seven out of ten pigs died within six days after inoculation. The mortality rate in the group of pigs inoculated with the lower dose was only two out of ten and, there were several pigs in this group that showed signs of respiratory distress besides some mild nervous signs. Pseudorabies virus was isolated from various tissues collected postmortem, including alveolar macrophages. Virus localization in tissues was also detected by in situ hybridization. The histopathological examination of the respiratory tract tissues revealed a pathological process that was progressing from mild pneumonia to severe suppurative bronchopneumonia. The isolation of virus from alveolar macrophages provides support to the hypothesis that replication of PRV during the course of infection produces an impairment of the defense mechanisms in the respiratory tract.
为了研究伪狂犬病病毒（PRV）在猪呼吸道疾病中的潜在作用，将9周龄的猪经鼻接种PRV毒株4892。使用了两种感染剂量：10(4.5)半数组织培养感染剂量（TCID50）/头猪和10(3.5) TCID50/头猪，每组10头猪。在接种10(4.5) TCID50的猪组中，10头猪中有7头在接种后6天内死亡。接种较低剂量的猪组死亡率仅为10头中的2头，并且该组中有几头猪除了出现一些轻度神经症状外，还表现出呼吸窘迫的迹象。从包括肺泡巨噬细胞在内的死后采集的各种组织中分离出伪狂犬病病毒。还通过原位杂交检测了病毒在组织中的定位。呼吸道组织的组织病理学检查显示了一个从轻度肺炎发展到严重化脓性支气管肺炎的病理过程。从肺泡巨噬细胞中分离出病毒为以下假设提供了支持，即PRV在感染过程中的复制会损害呼吸道的防御机制。
