Supanaranond K, Takeda N, Yamazaki S
Central Virus Diagnostic Laboratory, National Institute of Health, Tokyo, Japan.
Virus Genes. 1992 Apr;6(2):149-58. doi: 10.1007/BF01703064.
The complete nucleotide sequence was determined for the cDNAs that represent the RNA genome of the standard strain of a variant of coxsackievirus A24, the EH24/70, one of the agents causing acute hemorrhagic conjunctivitis. The genome is 7461 nucleotide long and is polyadenylated at the 3'-end terminus. Following a 750-nucleotide 5'-noncoding region, there was a long open reading frame of 6642 nucleotides, which serve to encode a viral polyprotein consisting of 2214 amino acids. Comparison of the deduced amino acid sequence of the polyprotein with those of known enteroviruses allowed us to predict the possible cleavage sites. The overall structure and the organization of the RNA genome is typical for an enterovirus. Based on the similarity of the nucleotide sequence of the 5' and 3' noncoding regions, together with the amino-acid sequence of the encoded proteins, EH24/70 appeared to be closely related to polioviruses and coxsackievirus A21.
测定了代表柯萨奇病毒A24变异体标准株EH24/70（急性出血性结膜炎致病因子之一）RNA基因组的cDNA的完整核苷酸序列。该基因组长度为7461个核苷酸，在3'-末端有polyA尾。在750个核苷酸的5'-非编码区之后，有一个6642个核苷酸的长开放阅读框，用于编码由2214个氨基酸组成的病毒多聚蛋白。将推导的多聚蛋白氨基酸序列与已知肠道病毒的序列进行比较，使我们能够预测可能的切割位点。RNA基因组的整体结构和组织是肠道病毒的典型特征。基于5'和3'非编码区核苷酸序列的相似性以及所编码蛋白质的氨基酸序列，EH24/70似乎与脊髓灰质炎病毒和柯萨奇病毒A21密切相关。
