Ryan M D, Jenkins O, Hughes P J, Brown A, Knowles N J, Booth D, Minor P D, Almond J W
A.F.R.C. Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, U.K.
J Gen Virol. 1990 Oct;71 ( Pt 10):2291-9. doi: 10.1099/0022-1317-71-10-2291.
Enterovirus type 70 (EV70) is the causative agent of acute haemorrhagic conjunctivitis and may also give rise to a rare neurological complication closely resembling poliomyelitis. The complete nucleotide sequence of the genome of EV70 has been determined from cDNA cloned in Escherichia coli. The genome consists of a 5' non-coding region of 726 nucleotides (nt), a long open reading frame of 6582 nt and a 3' non-coding region of 82 nt prior to the poly(A) tract. Comparison of the nucleotide sequence and the predicted amino acid sequence of the polyprotein with those published for other enteroviruses reveals sufficiently high similarity to predict antigenic regions and polyprotein cleavage sites. The P1 region of EV70 is as similar to those of the entero- as to those of the rhinoviruses, whereas the P2 and P3 regions are more closely related to the coxsackie B and swine vesicular disease viruses than other entero- or rhinoviruses.
肠道病毒70型（EV70）是急性出血性结膜炎的病原体，也可能引发一种极为罕见的、与脊髓灰质炎极为相似的神经并发症。EV70基因组的完整核苷酸序列已通过克隆于大肠杆菌中的cDNA得以确定。该基因组由一个726个核苷酸（nt）的5'非编码区、一个6582 nt的长开放阅读框以及位于多聚腺苷酸（poly(A)）序列之前的一个82 nt的3'非编码区组成。将该多聚蛋白的核苷酸序列和预测的氨基酸序列与其他肠道病毒已发表的序列进行比较，结果显示出足够高的相似性，从而能够预测抗原区域和多聚蛋白的切割位点。EV70的P1区域与肠道病毒以及鼻病毒的P1区域相似程度相当，而P2和P3区域与柯萨奇B病毒和猪水疱病病毒的关系比与其他肠道病毒或鼻病毒更为密切。
