Miyamura K, Takeda N, Tanimura M, Ogino T, Yamazaki S, Chen C W, Lin K H, Lin S Y, Ghafoor A, Yin-Murphy M
Central Virus Diagnostic Laboratory, National Institute of Health, Tokyo, Japan.
Arch Virol. 1990;114(1-2):37-51. doi: 10.1007/BF01311010.
The evolution of the variant of Coxsackievirus A 24 (CA 24 v) which causes acute hemorrhagic conjunctivitis was explored. Using 15 isolates obtained from Southeast Asia during the period 1970-1986, the genetic distance between isolates was estimated from pairwise comparison of nucleotide changes deduced from common spots on oligonucleotide maps of the isolates. From regression analysis of the genetic distance and the time of isolation of the isolates, the evolutionary rate of CA 24 v was estimated to be 3.44 x 10(-4)/nucleotide/month. The phylogenetic relationship of these isolates was explored using the neighbor-joining method and the modified unweighted pair group method using arithmetic averages (UPGMA). The phylogenetic tree constructed indicates that CA 24 v appeared from one focal place in July 1968 +/- 25 months, very close to the time of the first world epidemic of, then newly recognized, acute hemorrhagic conjunctivitis.
对引起急性出血性结膜炎的柯萨奇病毒A24型（CA 24 v）变种的进化情况进行了探究。利用1970年至1986年期间从东南亚获得的15株病毒分离株，通过对从分离株寡核苷酸图谱上的共同斑点推导得出的核苷酸变化进行成对比较，估算了分离株之间的遗传距离。根据遗传距离与分离株分离时间的回归分析，估计CA 24 v的进化速率为3.44×10⁻⁴/核苷酸/月。使用邻接法和改进的算术平均非加权配对组方法（UPGMA）探究了这些分离株的系统发育关系。构建的系统发育树表明，CA 24 v于1968年7月±25个月从一个疫源地出现，这与当时新发现的急性出血性结膜炎首次全球大流行的时间非常接近。
