Taherian Hanieh, Tafvizi Farzaneh, Fard Zahra Tahmasebi, Abdirad Afshin
Young Researchers and Elite Club, Islamic Azad University, Parand Branch, Parand, Iran.
Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran.
Prz Gastroenterol. 2014;9(5):280-4. doi: 10.5114/pg.2014.46163. Epub 2014 Oct 19.
Colorectal cancer is the third leading cause of cancer-related deaths worldwide, with nearly one million new cases identified annually. Different factors might cause colorectal cancer, one of the most prevalent cancers among both men and women. Viral aetiology in cancerous malignancies is a very important issue and so far a number of viral strains have been identified as tumour oncogene viruses. Viral infections, such as human papillomavirus (HPV), have recently been suggested as a risk factor for colorectal cancer. However, the aetiology of the disease is still unknown.
To assessed the association between HPV infection and colorectal cancer.
In this study, 50 cancer tissue samples and 50 samples without colon cancer were studied in order to identify HPV through polymerase chain reaction (PCR). Of 42 adenocarcinomas, 10 were well differentiated, 30 moderated differentiated, and 2 were poorly differentiated. DNA extraction was verified by beta globin gene amplification; specific PCR was carried out based on HPV L1 consensus primers MY09/MY11.
HPV DNA was not identified in any of the normal, adenocarcinoma, or adenoma samples.
In contrast with previous studies, the current research failed to establish a relationship between HPV infection and the incidence of colon cancer. Considering the existing inconsistencies, it is recommended that further studies be conducted with larger sample size.
结直肠癌是全球癌症相关死亡的第三大主要原因，每年有近100万新病例被确诊。不同因素可能导致结直肠癌，这是男性和女性中最常见的癌症之一。癌性恶性肿瘤中的病毒病因是一个非常重要的问题，到目前为止，一些病毒株已被确定为肿瘤致癌基因病毒。病毒感染，如人乳头瘤病毒（HPV），最近被认为是结直肠癌的一个风险因素。然而，该疾病的病因仍然未知。
评估HPV感染与结直肠癌之间的关联。
在本研究中，对50份癌组织样本和50份无结肠癌的样本进行研究，以便通过聚合酶链反应（PCR）鉴定HPV。在42例腺癌中，10例为高分化，30例为中分化，2例为低分化。通过β珠蛋白基因扩增验证DNA提取；基于HPV L1共有引物MY09/MY11进行特异性PCR。
在任何正常、腺癌或腺瘤样本中均未鉴定出HPV DNA。
与先前的研究相反，当前研究未能证实HPV感染与结肠癌发病率之间的关系。考虑到现有矛盾之处，建议进行更大样本量的进一步研究。
