de Gast G C, Boland G J, Vlieger A M, de Weger R A, Verdonck L F, Zwaan F E, Jiwa N M
Department of Hematology, University of Utrecht, The Netherlands.
Bone Marrow Transplant. 1992 Apr;9(4):221-5.
Infection with human cytomegalovirus (HCMV) after allogeneic bone marrow transplantation (BMT) was studied in 12 HCMV seronegative recipients of marrow from seropositive donors by weekly monitoring of cultures, expression of HCMV antigenemia (pp65) in granulocytes, polymerase chain reaction (PCR) on HCMV-DNA in granulocytes and IgM and IgG anti-HCMV antibodies. Eight patients remained negative in all tests as did 33 HCMV seronegative recipients of marrow from seronegative donors. In four patients, a transient expression of HCMV antigen pp65 in granulocytes from peripheral blood, together with a positive PCR on HCMV-DNA from the same samples were found without positive cultures, seroconversion or expression of other HCMV antigens in granulocytes. The data indicate the presence of an abortive HCMV infection in these four patients.
通过每周监测培养物、粒细胞中人类巨细胞病毒（HCMV）抗原血症（pp65）的表达、粒细胞中HCMV-DNA的聚合酶链反应（PCR）以及IgM和IgG抗HCMV抗体，对12名接受血清反应阳性供体骨髓的HCMV血清反应阴性的异基因骨髓移植（BMT）受者进行了HCMV感染研究。8名患者在所有检测中均呈阴性，33名接受血清反应阴性供体骨髓的HCMV血清反应阴性受者也是如此。在4名患者中，在外周血粒细胞中发现了HCMV抗原pp65的短暂表达，同时来自相同样本的HCMV-DNA的PCR检测呈阳性，但培养物未呈阳性、血清学转换或粒细胞中其他HCMV抗原的表达。数据表明这4名患者存在HCMV流产感染。
