人类疱疹病毒6与唾液腺中的内源性生物素
Human herpes virus 6 and endogenous biotin in salivary glands.
作者信息
Green M, Sviland L, Taylor C E, Peiris M, McCarthy A L, Pearson A D, Malcolm A J
机构信息
Department of Pathology, Royal Victoria Infirmary, Newcastle upon Tyne.
出版信息
J Clin Pathol. 1992 Sep;45(9):788-90. doi: 10.1136/jcp.45.9.788.
AIMS
To detect the presence of human herpes virus 6 (HHV6) and endogenous biotin in paraffin wax embedded and frozen salivary glands.
METHODS
Two stage indirect and streptavidin-biotin immunoperoxidase techniques were used to visualise the antigens.
RESULTS
HHV6 could not be shown in any of the tissues. However, considerable endogenous biotin antigenicity was detected in the glandular elements of the paraffin wax embedded material.
CONCLUSIONS
Results obtained with avidin-biotin detection systems should be interpreted with caution, especially when glandular epithelium is being stained. This may apply to both immunoperoxidase and in situ hybridisation techniques. The use of an anti-biotin antibody as a standard control should be considered.
目的
检测石蜡包埋和冷冻唾液腺中人类疱疹病毒6型（HHV6）及内源性生物素的存在情况。
方法
采用两步间接法及链霉亲和素-生物素免疫过氧化物酶技术来显示抗原。
结果
在任何组织中均未显示出HHV6。然而，在石蜡包埋材料的腺性成分中检测到了相当程度的内源性生物素抗原性。
结论
使用抗生物素蛋白-生物素检测系统获得的结果应谨慎解读，尤其是在对腺上皮进行染色时。这可能适用于免疫过氧化物酶技术和原位杂交技术。应考虑使用抗生物素抗体作为标准对照。
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