Foà-Tomasi L, Guerrini S, Huang T, Campadelli-Fiume G
Department of Experimental Pathology, University of Bologna, Italy.
Virology. 1992 Nov;191(1):511-6. doi: 10.1016/0042-6822(92)90222-b.
Monoclonal antibody 2D10 (MAb 2D10) raised toward human herpesvirus-6(U1102) [HHV6(U1102)] immunoprecipitated three glycosylated peptides, M(r) 112,000, 64,000, and 58,000, designated as gp112 from U1102-infected lymphocytes. Pulse-chase experiments suggest that the M(r) 64,000 and 58,000 polypeptides are very likely generated by post-translational cleavage of the M(r) 112,000 polypeptide. MAb 2D10 neutralized virion infectivity in the presence of complement, suggesting that gp112 is located in the virion envelope. MAb 2D10 did not prevent the appearance of HHV6-specific cytopathic effect. MAb 2D10 was reactive with denatured gp112 in immunoblots. HHV6 isolates form two clusters (Schimer, Wyatt, Yamanishi, Rodriguez, and Frenkel, Proc. Natl. Acad. Sci. USA 88, 5922; Ablashi, Balachandran, Josephs, Hung, Krtueger, Kramarsky, Salahuddin, and Gallo, Virology 184, 545). MAb 2D10 reacted by immunofluorescence and immunoprecipitation with the prototypes of each cluster, GS and Z29. Whereas the proteins immunoprecipitated by MAb 2D10 from GS-infected lymphocytes had an electrophoretic pattern very similar to that of U1102 gp112, the homologous glycoprotein immunoprecipitated from Z29-infected lymphocytes consisted of three polypeptides with M(r) 102,000, 59,000, and 50,000. The data suggest a variation among HHV6 isolates as far as this glycoprotein is concerned.
针对人疱疹病毒6型（U1102）[HHV6（U1102）]产生的单克隆抗体2D10（MAb 2D10）免疫沉淀出三条糖基化肽段，分子量分别为112,000、64,000和58,000，在U1102感染的淋巴细胞中被命名为gp112。脉冲追踪实验表明，分子量为64,000和58,000的多肽很可能是由分子量为112,000的多肽经翻译后切割产生的。MAb 2D10在补体存在的情况下中和了病毒体的感染性，表明gp112位于病毒体包膜中。MAb 2D10并没有阻止HHV6特异性细胞病变效应的出现。MAb 2D10在免疫印迹中与变性的gp112发生反应。HHV6分离株形成两个簇（希默、怀亚特、山岸、罗德里格斯和弗伦克尔，《美国国家科学院院刊》88, 5922；阿布拉希、巴拉昌德兰、约瑟夫斯、洪、克鲁格、克拉马尔斯基、萨拉胡丁和加洛，《病毒学》184, 545）。MAb 2D10通过免疫荧光和免疫沉淀与每个簇的原型GS和Z29发生反应。虽然MAb 2D10从GS感染的淋巴细胞中免疫沉淀出的蛋白质的电泳图谱与U1102 gp112非常相似，但从Z29感染的淋巴细胞中免疫沉淀出的同源糖蛋白由分子量为102,000、59,000和50,000的三条多肽组成。就这种糖蛋白而言，数据表明HHV6分离株之间存在差异。
