Foà-Tomasi L, Boscaro A, di Gaeta S, Campadelli-Fiume G
Department of Experimental Pathology, University of Bologna, Italy.
J Virol. 1991 Aug;65(8):4124-9. doi: 10.1128/JVI.65.8.4124-4129.1991.
We report the derivation and properties of a monoclonal antibody (MAb 2E4) which neutralizes human herpesvirus 6 (HHV-6). MAb 2E4 precipitated from lysates of infected cells a glycosylated polypeptide 100,000 in apparent molecular weight and minor components of 80,000, and 32,500. The predominant reactive protein after a pulse was the 100,000-molecular-weight peptide designated as gp100. The smaller polypeptides appeared in the precipitate predominantly after a chase. MAb 2E4 neutralized HHV-6 infectivity in the presence and in the absence of complement, and it inhibited the penetration of virus into the cells. Addition of MAb 2E4 as late as 6 h postinfection inhibited the formation of large polykaryocytes typical of HHV-6-infected cells.
我们报告了一种可中和人类疱疹病毒6型（HHV-6）的单克隆抗体（MAb 2E4）的衍生及特性。MAb 2E4从感染细胞的裂解物中沉淀出一种表观分子量为100,000的糖基化多肽以及分子量为80,000和32,500的次要成分。脉冲标记后主要的反应性蛋白是分子量为100,000的肽，命名为gp100。较小的多肽主要在追踪标记后出现在沉淀中。MAb 2E4在有补体和无补体存在的情况下均可中和HHV-6的感染性，并且它可抑制病毒进入细胞。感染后6小时加入MAb 2E4可抑制HHV-6感染细胞典型的大型多核细胞的形成。
