Foà-Tomasi L, Avitabile E, Campadelli-Fiume G
Department of Experimental Pathology, University of Bologna, Italy.
J Virol Methods. 1995 Feb;51(2-3):289-96. doi: 10.1016/0166-0934(94)00120-6.
A monoclonal antibody, designated as MAb 6E2, specific for human herpesvirus 6 variant B (HHV-6B) was derived from the spleen of a mouse immunized with lysates of HHV-6B(Z29) cord blood mononuclear cells. MAb 6E2 reacts by immunofluorescence with all the HIV-6B strains tested (Z29, CV, Hashimoto and SF) and fails to react with variant A prototypes, GS and U1102. The immunofluorescence staining was punctate and localized to the cytoplasm. The protein reacting with MAb 6E2 was identified as protein 48,000 in apparent M(r) value by immunoaffinity chromatography of lysates of HHV-6B-infected mononuclear cells.
一种名为单克隆抗体6E2的抗体，对人类疱疹病毒6型B亚型（HHV-6B）具有特异性，它源自用HHV-6B（Z29）脐血单核细胞裂解物免疫的小鼠脾脏。单克隆抗体6E2通过免疫荧光与所有测试的HHV-6B毒株（Z29、CV、桥本和SF）发生反应，而不与A亚型原型毒株GS和U1102发生反应。免疫荧光染色呈点状，定位于细胞质。通过对HHV-6B感染的单核细胞裂解物进行免疫亲和层析，与单克隆抗体6E2反应的蛋白质被鉴定为表观分子量为48,000的蛋白质。
