Li G, Li M, Ma J
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Wei Sheng Wu Xue Bao. 1992 Feb;32(1):63-7.
For researching the biosynthesis labelled Fusarin C(Fc) by Fuscarium moniliforme, a more quick and convenient method of Fusarin C production and purification were established, and a good liquid culture medium consisted of different kinds organic matters (hydroxy proline, sucrose and glycerin), inorganic salts and perlite replaced corn grit medium. The perlite-liquid culture medium inoculated with the strain of F. moniliforme yields 936mg Fc/kg organic matter with in 14 days of incubation at 28 degrees C. As compared with the corn grit medium, the amount of Fc from perlite-liquid medium was more than that from corn grit medium (831mg Fc/kg corn grit). In all experiments both thin-layer chromatography and high-pressure liquid chromatography were used to confirm the presence of Fc. parameters which were important for the optimal biosynthesis of Fc included hydroxy proline and sucrose concentrations, incubated time/temperature and amount of perlite. The 40g of sucrose/L liquid culture was optimal concentration for Fusarin C production. Of three contained N-matter tested, hydroxy proline was the best sources of N-atom for Fusarin C. Under the absence of hydroxy proline, the Fc wasn't synthesized in perlite-liquid culture medium by F. moniliforme. A culture time/temperature study of Fc production was done, and the optimal Fc amounts was synthesized after incubation for 14 days at 28 degrees C on perlite-liquid culture medium.
为研究串珠镰刀菌生物合成标记的镰刀菌素C（Fc），建立了一种更快速便捷的镰刀菌素C生产与纯化方法，并用由不同种类有机物（羟脯氨酸、蔗糖和甘油）、无机盐和珍珠岩组成的良好液体培养基替代了玉米粉培养基。接种串珠镰刀菌菌株的珍珠岩 - 液体培养基在28℃培养14天内每千克有机物可产生936毫克Fc。与玉米粉培养基相比，珍珠岩 - 液体培养基产生的Fc量高于玉米粉培养基（每千克玉米粉831毫克Fc）。在所有实验中，均使用薄层色谱法和高压液相色谱法来确认Fc的存在。对Fc最佳生物合成重要的参数包括羟脯氨酸和蔗糖浓度、培养时间/温度以及珍珠岩用量。每升液体培养基中40克蔗糖是生产镰刀菌素C的最佳浓度。在所测试的三种含氮物质中，羟脯氨酸是镰刀菌素C最佳的氮原子来源。在没有羟脯氨酸的情况下，串珠镰刀菌在珍珠岩 - 液体培养基中无法合成Fc。对Fc生产进行了培养时间/温度研究，在珍珠岩 - 液体培养基上于28℃培养14天后合成的Fc量最佳。
