Hsieh T T, Yao D S, Sheen I S, Liaw Y F, Pao C C
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Taipei, Taiwan, Republic of China.
Am J Clin Pathol. 1992 Oct;98(4):392-6. doi: 10.1093/ajcp/98.4.392.
RNA extracted from plasma and peripheral blood mononuclear cells of patients with chronic hepatitis C were used as the template for reverse transcription followed by double in vitro enzymatic amplification with nested primers. Hepatitis C virus was detected in 14 of 15 (93.3%) plasma specimens and in 8 of 15 (53.3%) peripheral blood mononuclear cell specimens obtained from patients with chronic hepatitis C and abnormal liver functions. The results suggest that hepatitis C virus could be found frequently in peripheral blood mononuclear cells of patients with chronic hepatitis C. Whether the presence of hepatitis C virus in peripheral blood mononuclear cells plays any role in the pathogenesis of diseases associated with hepatitis C virus infection remains to be determined.
从慢性丙型肝炎患者的血浆和外周血单核细胞中提取的RNA被用作逆转录模板，随后用巢式引物进行双重体外酶促扩增。在15份血浆标本中的14份（93.3%）以及从肝功能异常的慢性丙型肝炎患者获得的15份外周血单核细胞标本中的8份（53.3%）中检测到丙型肝炎病毒。结果表明，慢性丙型肝炎患者的外周血单核细胞中可频繁检测到丙型肝炎病毒。外周血单核细胞中丙型肝炎病毒的存在是否在丙型肝炎病毒感染相关疾病的发病机制中发挥任何作用仍有待确定。
