Young K C, Chang T T, Liou T C, Wu H L
Department of Medical Technology, National Cheng Kung University, Tainan, Taiwan, Republic of China.
J Med Virol. 1993 Sep;41(1):55-60. doi: 10.1002/jmv.1890410112.
The nested polymerase chain reaction (PCR) technique was applied to investigate hepatitis C virus (HCV) RNA in the peripheral blood mononuclear cells (PBMCs), saliva, and serum of patients with chronic type C hepatitis. The specificity of the amplified products was analyzed and confirmed by agarose gel electrophoresis, Southern blot hybridization, and restriction endonuclease pattern analysis. HCV RNA was detectable in the PBMCs of 24% (12/50) of the patients. The HCV RNA detected in PBMCs was not due to the contamination from plasma, since no viral sequences could be detected in the third washing of PBMCs. Of the 12 patients with HCV RNA in PBMCs, five were negative for HCV RNA sequences in the serum. Thus the presence of HCV RNA in PBMCs was not strictly correlated to the results for sera. Among 25 patients with HCV RNA in their saliva, 18 were negative for PBMCs. Among 25 patients without HCV RNA in their saliva, five had HCV RNA in PBMCs. In conclusion, PBMCs are an extrahepatic target for chronic HCV infection. However, we do not suggest that PBMCs act as a vehicle for carrying HCV to saliva, since the presence of HCV RNA in PBMCs and in saliva was not closely correlated.
采用巢式聚合酶链反应（PCR）技术检测慢性丙型肝炎患者外周血单个核细胞（PBMC）、唾液及血清中的丙型肝炎病毒（HCV）RNA。通过琼脂糖凝胶电泳、Southern印迹杂交及限制性内切酶图谱分析对扩增产物的特异性进行分析和确认。24%（12/50）的患者PBMC中可检测到HCV RNA。PBMC中检测到的HCV RNA并非血浆污染所致，因为在PBMC的第三次洗涤液中未检测到病毒序列。在12例PBMC中检测到HCV RNA的患者中，5例血清HCV RNA序列为阴性。因此，PBMC中HCV RNA的存在与血清检测结果并不严格相关。在25例唾液中检测到HCV RNA的患者中，18例PBMC为阴性。在25例唾液中未检测到HCV RNA的患者中，5例PBMC中有HCV RNA。总之，PBMC是慢性HCV感染的肝外靶细胞。然而，我们并不认为PBMC是HCV传播至唾液的载体，因为PBMC和唾液中HCV RNA的存在并无密切关联。
