Zignego A L, Macchia D, Monti M, Thiers V, Mazzetti M, Foschi M, Maggi E, Romagnani S, Gentilini P, Bréchot C
Hybridotest, Institut Pasteur, Paris, France.
J Hepatol. 1992 Jul;15(3):382-6. doi: 10.1016/0168-8278(92)90073-x.
We investigated the infection of peripheral blood mononuclear cells (PBMNC) by hepatitis C virus (HCV) in 5 patients with HCV-related chronic hepatitis. The presence of HCV-RNA-positive and -negative strands was tested with the polymerase chain reaction (PCR) method. In all subjects, HCV-RNA was shown in PBMNC. In 3 cases, HCV-RNA was shown in the T- and B-cell populations, with viral RNA also present in the monocyte-macrophage fraction of two of these. HCV-RNA-negative stranded molecules, indicative of the viral multiplication, were significantly increased in cells maintained in cultures with PHA/PMA stimulation. The results indicate that HCV infect blood mononuclear cells, thus suggesting that this cellular tropism may play a role in HCV infection.
我们对5例丙型肝炎病毒（HCV）相关慢性肝炎患者的外周血单个核细胞（PBMNC）感染情况进行了研究。采用聚合酶链反应（PCR）方法检测HCV - RNA阳性和阴性链的存在情况。在所有受试者的PBMNC中均检测到HCV - RNA。在3例患者中，T细胞和B细胞群体中均检测到HCV - RNA，其中2例患者的单核细胞 - 巨噬细胞部分也存在病毒RNA。在PHA/PMA刺激培养的细胞中，指示病毒增殖的HCV - RNA负链分子显著增加。结果表明HCV可感染血液单核细胞，提示这种细胞嗜性可能在HCV感染中起作用。
