Yamate J, Tajima M, Shibuya K, Saitoh T, Kizaki H
Nippon Institute for Biological Science, Tokyo, Japan.
J Comp Pathol. 1992 Jul;107(1):59-72. doi: 10.1016/0021-9975(92)90096-d.
A transplantable tumour, designated NB-Y, was established from a spontaneous nephroblastoma in an F344 rat. NB-Y was serially passaged in syngeneic rats by subcutaneous implantation up to the 49th generation. The transplants grew into nodules with an average diameter of 5 cm and average weight of 92.9 g 4 weeks after implantation. The primary tumour and NB-Y consisted mainly of sheets or clusters of undifferentiated blastemal cells, which reacted immunohistochemically for vimentin but not for keratin. Renin-containing cells were observed in the small blood vessel walls within the primary tumour, but neoplastic cells of both primary tumour and NB-Y failed to stain for renin. Plasma renin activity was significantly higher (40.7 ng per ml per h) in transplanted rats 4 weeks after implantation compared with non-transplanted controls (28.0 ng per ml per h). Hyperplastic juxtaglomerular cells were often observed in rats bearing NB-Y. Sinusoidal dilatation was present in the liver, adrenal glands, pituitary gland and bone marrow of recipients, suggesting abnormal blood flow provoked via the renin-angiotensin system. The present study revealed the development of hyper-reninaemia in NB-Y-bearing rats, but its pathogenesis remains unknown.
一种可移植肿瘤，命名为NB-Y，是从一只F344大鼠的自发性肾母细胞瘤中培育出来的。NB-Y通过皮下植入在同基因大鼠中连续传代至第49代。植入后4周，移植瘤长成平均直径为5厘米、平均重量为92.9克的结节。原发性肿瘤和NB-Y主要由未分化的胚基细胞片或细胞簇组成，这些细胞免疫组化检测波形蛋白呈阳性反应，而细胞角蛋白呈阴性反应。在原发性肿瘤的小血管壁中观察到含肾素的细胞，但原发性肿瘤和NB-Y的肿瘤细胞肾素染色均为阴性。与未移植的对照组（28.0纳克/毫升/小时）相比，植入后4周的移植大鼠血浆肾素活性显著更高（40.7纳克/毫升/小时）。在携带NB-Y的大鼠中经常观察到肾小球旁细胞增生。受体的肝脏、肾上腺、垂体和骨髓出现血窦扩张，提示通过肾素-血管紧张素系统引发了异常血流。本研究揭示了携带NB-Y的大鼠出现高肾素血症，但发病机制尚不清楚。
