Ward R L, Clemens J D, Knowlton D R, Rao M R, van Loon F P, Huda N, Ahmed F, Schiff G M, Sack D A
Division of Clinical Virology, J. N. Gamble Institute of Medical Research, Cincinnati, Ohio 45219.
J Infect Dis. 1992 Dec;166(6):1251-7. doi: 10.1093/infdis/166.6.1251.
This case-control study sought to determine whether protection against clinically significant rotavirus diarrhea in children aged 4-35 months correlated with titers of serum neutralizing antibody and, if so, whether this protection was serotype-specific. Titers of acute-phase sera from 156 cases of treated rotavirus diarrhea in rural Bangladesh were contrasted with titers from 312 contemporaneously selected, age-matched controls. Analyses of the culture-adapted rotaviruses from the cases revealed that 24%, 15%, 43%, and 17% belonged to serotypes 1-4, respectively. Titers of both homologous and heterologous neutralizing antibody in acute blood specimens of cases were significantly lower than those of matched controls. However, multivariate logistic regression models demonstrated that only antibody titers to heterotypic rotaviruses were independently associated with protection against rotavirus disease. These data, which indicate that the correlation of protection with neutralizing antibody titers is not serotype-specific, suggest that immunity to rotavirus disease may be mediated by other factors.
这项病例对照研究旨在确定4至35个月大儿童针对临床上显著的轮状病毒腹泻的保护作用是否与血清中和抗体滴度相关，若相关，这种保护作用是否具有血清型特异性。将孟加拉国农村地区156例接受治疗的轮状病毒腹泻病例的急性期血清滴度与同期选取的312例年龄匹配的对照的滴度进行对比。对病例中经培养适应的轮状病毒分析显示，分别有24%、15%、43%和17%属于血清型1至4。病例急性期血液标本中同源和异源中和抗体的滴度均显著低于匹配对照的滴度。然而，多变量逻辑回归模型表明，只有针对异型轮状病毒的抗体滴度与预防轮状病毒疾病独立相关。这些数据表明保护作用与中和抗体滴度的相关性并非血清型特异性，提示针对轮状病毒疾病的免疫可能由其他因素介导。
