Wolf D G, Spector S A
Department of Pediatrics, University of California, San Diego 92093.
J Infect Dis. 1992 Dec;166(6):1412-5. doi: 10.1093/infdis/166.6.1412.
The utility of amplification of human cytomegalovirus (HCMV) DNA in cerebrospinal fluid (CSF) for the diagnosis of HCMV central nervous system (CNS) disease in AIDS patients was studied. CSF specimens from 30 patients with neurologic dysfunction were assayed by polymerase chain reaction (PCR), and the results were correlated with histopathologic findings, CSF culture, and clinical manifestations. PCR was positive in all 11 patients who had histopathologic evidence of HCMV CNS disease, including 4 who were CSF culture-negative. Three patients with HCMV polyradiculopathy had CSF positive by PCR. Nine patients negative for HCMV by neuropathologic study and an additional 7 patients with HCMV-unrelated clinical diagnosis were all CSF PCR-negative, despite concomitant systemic HCMV infection in 7. In addition, 24 asymptomatic human immunodeficiency virus-infected individuals were CSF PCR-negative. CSF PCR appears to be a sensitive and specific diagnostic method for detection of HCMV CNS disease in AIDS patients.
研究了脑脊液（CSF）中人类巨细胞病毒（HCMV）DNA扩增在艾滋病患者HCMV中枢神经系统（CNS）疾病诊断中的效用。对30例有神经功能障碍的患者的CSF标本进行聚合酶链反应（PCR）检测，并将结果与组织病理学发现、CSF培养及临床表现进行关联分析。11例有HCMV CNS疾病组织病理学证据的患者PCR均为阳性，其中4例CSF培养阴性。3例患有HCMV多发性神经根病的患者CSF经PCR检测为阳性。9例经神经病理学研究HCMV阴性的患者以及另外7例临床诊断与HCMV无关的患者，尽管其中7例同时存在全身性HCMV感染，但CSF PCR均为阴性。此外，24例无症状的人类免疫缺陷病毒感染个体CSF PCR也为阴性。CSF PCR似乎是检测艾滋病患者HCMV CNS疾病的一种敏感且特异的诊断方法。
