三种用于诊断巨细胞病毒性脑炎的脑脊液核酸扩增检测方法的比较
Comparison of three nucleic acid amplification assays of cerebrospinal fluid for diagnosis of cytomegalovirus encephalitis.
作者信息
Bestetti A, Pierotti C, Terreni M, Zappa A, Vago L, Lazzarin A, Cinque P
机构信息
Division of Infectious Diseases, San Raffaele Hospital, Via Stamira d'Ancona 20, 20127 Milan, Italy.
出版信息
J Clin Microbiol. 2001 Mar;39(3):1148-51. doi: 10.1128/JCM.39.3.1148-1151.2001.
Abstract
The diagnostic reliabilities of three cytomegalovirus (CMV) nucleic acid amplification assays of cerebrospinal fluid (CSF) were compared by using CSF samples from human immunodeficiency virus-infected patients with a postmortem histopathological diagnosis of CMV encephalitis (n = 15) or other central nervous system conditions (n = 16). By using a nested PCR assay, the quantitative COBAS AMPLICOR CMV MONITOR PCR, and the NucliSens CMV pp67 nucleic acid sequence-based amplification assay, sensitivities were 93.3, 86.6, and 93.3%, respectively, and specificities were 93.7, 93.7, and 87.5%, respectively. The COBAS AMPLICOR assay revealed significantly higher CMV DNA levels in patients with diffuse ventriculoencephalitis than in patients with focal periventricular lesions.
摘要
通过使用来自人类免疫缺陷病毒感染患者的脑脊液（CSF）样本，比较了三种脑脊液巨细胞病毒（CMV）核酸扩增检测方法的诊断可靠性，这些患者经尸检组织病理学诊断为CMV脑炎（n = 15）或其他中枢神经系统疾病（n = 16）。使用巢式PCR检测、定量COBAS AMPLICOR CMV MONITOR PCR和基于核酸序列扩增的NucliSens CMV pp67检测，敏感性分别为93.3%、86.6%和93.3%，特异性分别为93.7%、93.7%和87.5%。COBAS AMPLICOR检测显示，弥漫性脑室脑炎患者的CMV DNA水平显著高于局灶性脑室周围病变患者。
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