Del Prete Chiara, Calabria Alfonso, Longobardi Valentina, Palumbo Veronica, Merlo Barbara, Iacono Eleonora, Tafuri Simona, Carotenuto Domenico, Ciani Francesca, Damiano Sara, Ciarcia Roberto, Cocchia Natascia
Department of Veterinary Medicine and Animal Productions, University of Naples 'Federico II', Via Federico Delpino 1, 80137 Naples, Italy.
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
Animals (Basel). 2022 Jun 26;12(13):1638. doi: 10.3390/ani12131638.
Antioxidant supplementation has been proposed as a new strategy to improve the long-term preservation of semen. The aim of this study was to evaluate the effect of Maca supplementation of semen extender on quality-related canine semen parameters during cooling. Ejaculates from nine dogs were cooled for 7 days in the absence (control group) or in the presence of 10, 20 and 50 μL/mL of an aqueous extract of Maca. Sperm were evaluated for sperm viability, motility, DNA fragmentation and lipid peroxidation after 3 h, 24 h, 4 days and 7 days of storage. The addition of 10 μL/mL of Maca preserved sperm DNA and plasma membrane integrity at 3 h and increased sperm curvilinear velocity after 24 h. Treatment with 20 and 50 μL/mL of Maca increased the percentage of hyperactivated sperm after 3 h. Moreover, semen treated with 20 μL/mL of Maca decreased lipid peroxidation at 24 h. A significant reduction of sperm DNA and plasma membrane integrity as well as of kinetics parameters between 3 and 24 h of refrigerated storage with the higher concentration tested was observed. Although Maca was not able to protect canine semen with extended refrigeration storage time, it increased hyperactivation and preserved DNA integrity in short-term storage.
抗氧化剂补充已被提议作为一种改善精液长期保存的新策略。本研究的目的是评估在精液稀释液中添加玛咖对犬精液在冷却过程中与质量相关参数的影响。采集9只犬的精液，分别在无玛咖（对照组）以及添加10、20和50 μL/mL玛咖水提取物的情况下冷却7天。在储存3小时、24小时、4天和7天后，对精子的活力、运动能力、DNA碎片化和脂质过氧化进行评估。添加10 μL/mL玛咖可在3小时时保持精子DNA和质膜完整性，并在24小时后提高精子曲线速度。添加20和50 μL/mL玛咖可在3小时后增加超活化精子的百分比。此外，添加20 μL/mL玛咖的精液在24小时时脂质过氧化减少。在测试的较高浓度下，观察到冷藏储存3至24小时期间精子DNA和质膜完整性以及动力学参数显著降低。虽然玛咖不能在延长冷藏储存时间的情况下保护犬精液，但它在短期储存中增加了超活化并保持了DNA完整性。
