Kellokoski J K, Syrjänen S M, Chang F, Yliskoski M, Syrjänen K J
Department of Oral Pathology, Kuopio University Hospital, Finland.
J Oral Pathol Med. 1992 Nov;21(10):459-64. doi: 10.1111/j.1600-0714.1992.tb00975.x.
The presence of human papillomavirus (HPV) in biopsies taken from clinically normal buccal mucosa (n = 212) and clinical lesions (n = 60) was examined by Southern blot hybridization (SBH) using 32P-labelled HPV DNA probes. Furthermore, one hundred formalin-fixed, paraffin-embedded biopsies were analyzed by using polymerase chain reaction (PCR), combined with dot blot hybridization and biotinylated HPV DNA probes. With SBH and PCR, 15.4% and 29.4% of the biopsies, respectively, contained HPV DNA. In clinically normal epithelium, 15.6% and 23.1% of the samples were HPV-positive with SBH and PCR, respectively. The HPV types detected in the genital and oral mucosa of index patients differed in all except two cases. Histology could not be relied on distinguishing HPV DNA positive and HPV DNA negative samples. Hand warts were encountered significantly more frequently in patients with a concomitant oral HPV infection. To conclude, oral HPV infections as detected by SBH and PCR are surprisingly common, but similar to the genital tract, the virus seems to exist in a latent form in the vast majority of cases. The frequent concomitant finding of skin warts and oral HPV infection may suggest some kind of HPV-specific immunosuppression.
采用32P标记的人乳头瘤病毒（HPV）DNA探针，通过Southern印迹杂交（SBH）检测从临床正常颊黏膜（n = 212）和临床病变（n = 60）获取的活检样本中HPV的存在情况。此外，使用聚合酶链反应（PCR）结合斑点印迹杂交和生物素化HPV DNA探针，对100份福尔马林固定、石蜡包埋的活检样本进行分析。采用SBH和PCR检测时，分别有15.4%和29.4%的活检样本含有HPV DNA。在临床正常上皮组织中，采用SBH和PCR检测时，分别有15.6%和23.1%的样本为HPV阳性。除两例外，索引患者生殖器和口腔黏膜中检测到的HPV类型均不同。组织学无法可靠地区分HPV DNA阳性和阴性样本。伴有口腔HPV感染的患者手部疣的发生率明显更高。总之，通过SBH和PCR检测到的口腔HPV感染惊人地常见，但与生殖道类似，在绝大多数情况下，病毒似乎以潜伏形式存在。皮肤疣与口腔HPV感染的频繁并发可能提示某种HPV特异性免疫抑制。
