Yeudall W A, Campo M S
Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, Glasgow, U.K.
J Gen Virol. 1991 Jan;72 ( Pt 1):173-6. doi: 10.1099/0022-1317-72-1-173.
The DNAs of human papillomavirus (HPV) types 4, 16 and 18 have been detected in biopsies of normal and malignant human oral mucosa by Southern blot hybridization and the polymerase chain reaction (PCR). By the former technique, HPV-4, HPV-16 and HPV-18 DNAs were detected in three separate carcinomas but were found in adjacent dysplastic and normal tissue by the PCR only. The PCR technique also allowed detection of HPV-16 and HPV-18 DNA in additional carcinomas and normal samples. The oral HPV-4 DNA was molecularly cloned and extensive restriction analysis and nucleotide sequencing showed identity with the prototype HPV-4 DNA. The HPV-18 DNA detected by Southern blot hybridization showed an altered restriction pattern in the E1 region of the viral genome; however direct nucleotide sequencing of PCR products from the E6 open reading frame showed no sequence alterations in either normal or malignant samples.
通过Southern印迹杂交和聚合酶链反应（PCR），在正常和恶性人类口腔黏膜活检组织中检测到了4型、16型和18型人乳头瘤病毒（HPV）的DNA。采用前一种技术，在3例不同的癌组织中检测到了HPV-4、HPV-16和HPV-18的DNA，但仅通过PCR在相邻的发育异常和正常组织中发现了这些病毒DNA。PCR技术还能在其他癌组织和正常样本中检测到HPV-16和HPV-18的DNA。对口腔HPV-4 DNA进行了分子克隆，广泛的限制性分析和核苷酸测序表明其与HPV-4原型DNA一致。通过Southern印迹杂交检测到的HPV-18 DNA在病毒基因组的E1区域显示出改变的限制性图谱；然而，来自E6开放阅读框的PCR产物的直接核苷酸测序表明，在正常或恶性样本中均未发现序列改变。
