Park J H, Ochiai K, Itakura C
Department of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Comp Pathol. 1992 Oct;107(3):329-40. doi: 10.1016/0021-9975(92)90008-i.
Liver tissues from rabbits experimentally infected with rabbit haemorrhagic disease virus (RHDV) were studied electron microscopically. The earliest change in hepatocytes of the rabbits infected with RHDV was hydropic degeneration. Rough endoplasmic reticulum was dilated with a mild increase in polysomes and cytoplasmic cisternae in degenerated hepatocytes. Characteristic cytopathological changes of necrotic hepatocytes included shrinkage of the cell body, formation of cytoplasmic vesicles, vacuoles or cisternae and karyolysis. A large number of viral particles resembling a calicivirus in size and morphology was demonstrated in the cytoplasm of many necrotic hepatocytes. The particles had accumulated mainly in the membrane-bound cisternae or scattered around the membrane-bound vacuoles of the necrotic hepatocytes. Western blot analysis demonstrated that RHDV antigen was present in the infected hepatocytes. RHDV particles were also detected by immunoelectron microscopy. Replicating patterns of RHDV particles and subsequent cytopathology resembled those in other calicivirus infections.
对经实验感染兔出血症病毒（RHDV）的家兔肝脏组织进行了电子显微镜研究。感染RHDV的家兔肝细胞最早出现的变化是水样变性。粗面内质网扩张，变性肝细胞中的多核糖体和胞质池轻度增加。坏死肝细胞的特征性细胞病理学变化包括细胞体收缩、细胞质小泡、空泡或池的形成以及核溶解。在许多坏死肝细胞的细胞质中发现了大量大小和形态类似于杯状病毒的病毒颗粒。这些颗粒主要聚集在坏死肝细胞的膜结合池中或散布在膜结合空泡周围。蛋白质印迹分析表明，RHDV抗原存在于受感染的肝细胞中。免疫电子显微镜也检测到了RHDV颗粒。RHDV颗粒的复制模式及随后的细胞病理学与其他杯状病毒感染相似。
