HOWES D W, MELNICK J L, REISSIG M
J Exp Med. 1956 Sep 1;104(3):289-304. doi: 10.1084/jem.104.3.289.
A sequential study is reported of the cytological changes induced in cultures of monkey kidney epithelial cells by poliovirus. The pattern of cytological changes was followed through a single cycle of virus multiplication. Morphological alterations were correlated with the appearance of new infective virus within the cells and in the culture fluid. Alteration of the chromatin pattern of the nucleus, and Type B acidophilic intranuclear inclusions, were seen as early as 4 hours after virus inoculation. Later wrinkling and shrivelling of the nucleus occurred, and eosinophilic cytoplasmic masses appeared. The rounded, pycnotic cell, customarily used as an index of the cytopathic response, was found only during the last stages of the infective process. On the basis of these changes, infected cells could be classified into six different types. Differential cell counts were made on the stained cultures, and the stage of cytopathic degeneration was correlated with the appearance of virus in the cells and in the culture fluid. Newly formed virus could be detected within the infected cells at about the same time that the first nuclear alterations and intranuclear inclusions were seen. The virus-induced morphological changes exhibited a specificity distinct from the classical pycnosis of autolytic degeneration.
本文报道了一项关于脊髓灰质炎病毒对猴肾上皮细胞培养物所诱导的细胞学变化的连续性研究。通过病毒增殖的单个周期追踪细胞学变化模式。形态学改变与细胞内及培养液中新感染性病毒的出现相关。早在接种病毒4小时后，就可见细胞核染色质模式的改变以及B型嗜酸性核内包涵体。随后细胞核出现皱缩和干瘪，并出现嗜酸性细胞质团块。通常用作细胞病变反应指标的圆形、固缩细胞，仅在感染过程的最后阶段才出现。基于这些变化，感染细胞可分为六种不同类型。对染色培养物进行细胞分类计数，并将细胞病变退化阶段与细胞及培养液中病毒的出现相关联。在首次观察到细胞核改变和核内包涵体的大致同时，可在感染细胞内检测到新形成的病毒。病毒诱导的形态学变化表现出与自溶变性经典固缩不同的特异性。
