Ong T M, Serres F J
Genetics. 1975 Jul;80(3):475-82. doi: 10.1093/genetics/80.3.475.
The genetic characterization of ad-3 mutants of Neurospora crassa induced by two carcinogenic difunctional akylating agents, 1,2,4,5-diepoxypentane (DEP) and 1,2,7,8-diepoxyoctane (DEO), has shown that point mutations at the ad-3B locus have similar complementation patterns. In addition to the induction of point mutations, DEP induces a low frequency (7.5%) of multilocus deletions, whereas DEO induces an extremely high frequency (42.0%). The distribution of the different classes of ad-3 mutants and the frequency of multilocus deletion mutants among DEP-induced mutants are not significantly different from those induced by the monofunctional alkylating agents EI, EMS and ICR-177 at comparable forward-mutation frequencies. Moreover, the frequencies of DEP-induced ad-3B mutants showing allelic completion or having nonpolarized complementation patterns are similar to those of ad-3B mutants induced by monofunctional agents. It is suggested, therefore, that the mechanism of mutation-induction by DEP in N. crassa is similar to that of monofunctional alkylating agents. Mutation-induction by DEO probably results both from the mechanism of action of monofunctional alkylating agents and from inter-strand cross-linkage of the DNA molecular by the two functional epoxy groups.
对由两种致癌双功能烷基化剂1,2,4,5 - 二环氧戊烷（DEP）和1,2,7,8 - 二环氧辛烷（DEO）诱导的粗糙脉孢菌ad - 3突变体进行的遗传特征分析表明，ad - 3B位点的点突变具有相似的互补模式。除了诱导点突变外，DEP还诱导低频（7.5%）的多位点缺失，而DEO诱导的频率极高（42.0%）。在可比的正向突变频率下，DEP诱导的突变体中不同类别的ad - 3突变体的分布以及多位点缺失突变体的频率与单功能烷基化剂EI、EMS和ICR - 177诱导的情况没有显著差异。此外，DEP诱导的显示等位基因互补或具有非极化互补模式的ad - 3B突变体的频率与单功能试剂诱导的ad - 3B突变体相似。因此，有人提出，DEP在粗糙脉孢菌中诱导突变的机制与单功能烷基化剂相似。DEO诱导突变可能既源于单功能烷基化剂的作用机制，也源于DNA分子通过两个功能性环氧基团发生的链间交联。
