Anderson P, Brenner S
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4470-4. doi: 10.1073/pnas.81.14.4470.
The unc-54 gene of Caenorhabditis elegans encodes an abundant myosin heavy chain protein expressed in body-wall muscle cells. We have designed genetic techniques that select directly for unc-54 mutants. This selection is based upon properties of the unc-54 dominant allele e1152. Mutations that eliminate dominance of e1152 are null alleles of unc-54. Deletions have been identified by their genetic properties. We have defined mutationally a number of essential genes near unc-54, and we have described the genetic fine structure of this region of linkage group I. As much as 27% of the unc-54 mutations induced by the bifunctional alkylating agent 1,2,7,8-diepoxyoctane are multisite deletions. Extrachromosomal free duplications that include unc-54 are also described.
秀丽隐杆线虫的unc-54基因编码一种在体壁肌肉细胞中表达的丰富的肌球蛋白重链蛋白。我们设计了直接选择unc-54突变体的遗传技术。这种选择基于unc-54显性等位基因e1152的特性。消除e1152显性的突变是unc-54的无效等位基因。已通过其遗传特性鉴定出缺失。我们通过突变定义了unc-54附近的许多必需基因，并描述了连锁群I该区域的遗传精细结构。双功能烷基化剂1,2,7,8-二环氧辛烷诱导的unc-54突变中多达27%是多位点缺失。还描述了包含unc-54的染色体外游离重复序列。
