Krybus J, Bueno M A, Feder D, Andrade Júnior A F, Cabral M, Novak A, Martins M C
Department of Morphology and Physiology, Faculdade de Medicina do ABC, SP, Brasil.
Rev Paul Med. 1992 May-Jun;110(3):102-7.
to verify the effects of Listeria monocytogenes (LM) inoculation in the survival of animals bearing Ehrlich's tumor. KIND OF STUDY: experimental. Animals-isogenic mice, Balb/c, female, 19-21 g. Tumor-Ascitic Ehrlich's tumor, dilution of 5 x 10(5) cells/0.1 ml. Bacteria-LM serotype 4a, solution with 7 x 10(3) bacteria (standard sub-lethal dose). Intervention-a) inoculation of LM in mice bearing Ehrlich tumor at the same time as ascitic cells transplantation. b) inoculation of LM seven days before and, again, seven and fourteen days after ascitic cells transplantation. c) to study the effect of using ampicillin 100 mg/kg, im, simultaneously with the inoculation of Ehrlich tumor and LM organisms and, again, 3, 5, 7, 14, 21 and 30 days after the ascitic cells transplantation.
Chi-square test; p < 0.05
LM increases significantly the survival of mice bearing Ehrlich tumor even when only one inoculum of viable LM was used, seven days before or seven days after the ascitic cells transplantation. The use of ampicillin after the inoculation of LM and tumor transplantation does not alter the survival of mice.
验证接种单核细胞增生李斯特菌（LM）对荷艾氏腹水癌动物存活情况的影响。研究类型：实验性研究。动物——同基因雌性Balb/c小鼠，体重19 - 21克。肿瘤——艾氏腹水癌，5×10⁵个细胞/0.1毫升的稀释液。细菌——LM 4a血清型，含7×10³个细菌的溶液（标准亚致死剂量）。干预措施：a）在接种腹水细胞的同时给荷艾氏腹水癌小鼠接种LM。b）在接种腹水细胞前7天接种LM，在接种腹水细胞后7天和14天再次接种LM。c）研究在接种艾氏腹水癌和LM的同时，以及在接种腹水细胞后3、5、7、14、21和30天，肌肉注射100毫克/千克氨苄西林的效果。
卡方检验；p < 0.05
即使仅在接种腹水细胞前7天或后7天使用一次活的LM接种，LM也能显著提高荷艾氏腹水癌小鼠的存活率。在接种LM和肿瘤移植后使用氨苄西林不会改变小鼠的存活率。
