Bingen E, Denamur E, Lambert-Zechovsky N, Brahimi N, el Lakany M, Elion J
Laboratoire de Bactériologie, Hôpital Robert Debré, Paris, France.
J Hosp Infect. 1992 Jun;21(2):95-101. doi: 10.1016/0195-6701(92)90028-k.
Restriction fragment length polymorphism analysis (RFLP) of total DNA and rDNA regions was used for the epidemiological evaluation of 10 Enterobacter cloacae nosocomial isolates obtained from nine patients in our hospital. Five of these patients were hospitalized during overlapping periods, thus raising the question of cross-contamination. A single biochemical pattern and antibiotic susceptibility profile was observed for all isolates but one. In contrast, based on the results of total DNA and rDNA RFLP patterns, the genetic unrelatedness of the isolates was clearly shown, thus excluding a common source of contamination or patient-to-patient transfer.
采用总DNA和rDNA区域的限制性片段长度多态性分析（RFLP）对我院9例患者的10株阴沟肠杆菌医院分离株进行流行病学评估。其中5例患者在重叠期间住院，因此引发了交叉污染的问题。除1株分离株外，所有分离株均观察到单一的生化模式和抗生素敏感性谱。相反，基于总DNA和rDNA RFLP模式的结果，分离株的遗传不相关性清晰显示，从而排除了污染的共同来源或患者之间的传播。
