Wätzig V, Ruffert K, Güldner G
Allerg Immunol (Leipz). 1976;22(1):97-103.
For the demonstration of a cell-mediated sensitisation, tuberculin allergics, chromate allergics, penicillin allergics, and controls were investigated. The method used was the measurement of leukocyte migration inhibition in a Teflon skin chamber with and without addition of antigen, which approaches to testing under in vivo conditions. In 10 of 11 tuberculin allergics, 6 of 7 chromate allergics, and 7 penicillin allergics, a clear inhibition of leucocyte migration after addition of antigen could be observed (maximum 16 h after addition of antigen). In 16 unsensitised controls no significant migration inhibition appeared. Possible advantages of this method over in vitro methods and intracutaneous tests are discussed.
为了证明细胞介导的致敏作用，对结核菌素过敏者、铬酸盐过敏者、青霉素过敏者及对照组进行了研究。所采用的方法是在添加和不添加抗原的情况下，在聚四氟乙烯皮肤小室中测量白细胞迁移抑制情况，这近似于在体内条件下进行检测。在11名结核菌素过敏者中的10名、7名铬酸盐过敏者中的6名以及7名青霉素过敏者中，添加抗原后可观察到白细胞迁移明显受到抑制（添加抗原后最长16小时）。在16名未致敏的对照组中，未出现明显的迁移抑制。文中讨论了该方法相对于体外方法和皮内试验可能具有的优势。
