Han T, Dadey B
Immunology. 1976 Oct;31(4):643-8.
Four different combinations of one-way mixed lymphocyte reactions between human peripheral blood T and B lymphocytes (at a ratio of 1:1), purified by the E-rosetting technique, were carried our. A significant mixed lymphocyte reaction was observed only in a combination in which T lymphocytes, as responding cells, and B lymphocytes, as stimulating cells, were utilized. No significant mixed lymphocyte reaction was noted in the other three combinations of cells, using T or B lymphocytes as responders and T lymphocytes as stimulators, and also B lymphocytes as both responders and stimulators. Mixed lymphocyte reactions between T lymphocytes as responders (at constant concentration) and T and B lymphocytes as stimulators (varying proportions) showed that the response decreased proportionately with decreasing numbers of B cells and increasing numbers of T cells used as stimulators. Addition of increasing numbers of stimulating T cells to a constant number of stimulating B cells did not suppress or enhance the T-cell response to B cells. These observations indicate that the human peripheral blood T and B lymphocytes play an exclusive role as responding cells and stimulating cells, respectively.
采用E花环技术纯化人外周血T淋巴细胞和B淋巴细胞，以1:1的比例进行了四种不同组合的单向混合淋巴细胞反应。仅在以T淋巴细胞作为反应细胞、B淋巴细胞作为刺激细胞的组合中观察到显著的混合淋巴细胞反应。在其他三种细胞组合中，即分别以T淋巴细胞或B淋巴细胞作为反应细胞、T淋巴细胞作为刺激细胞，以及B淋巴细胞同时作为反应细胞和刺激细胞，均未观察到显著的混合淋巴细胞反应。以T淋巴细胞作为反应细胞（浓度恒定）、T淋巴细胞和B淋巴细胞作为刺激细胞（比例不同）的混合淋巴细胞反应表明，随着作为刺激细胞的B细胞数量减少和T细胞数量增加，反应相应降低。向恒定数量的刺激B细胞中添加越来越多的刺激T细胞，既不抑制也不增强T细胞对B细胞的反应。这些观察结果表明，人外周血T淋巴细胞和B淋巴细胞分别作为反应细胞和刺激细胞发挥独特作用。
