Fernandez L A, MacSween J M
Immunology. 1981 Dec;44(4):653-9.
The response of isolated T cells to autologous non-T mononuclear cells is called the autologous mixed lymphocyte reaction (AMLR). The responding T cells show immunological memory and specificity. This reaction was present in all thirty normal individuals tested. Since the AMLR in the absence of evidence of in vivo excessive lymphoproliferation must somehow be controlled, we have studied the interactions of enriched T cells, B cells and monocytes in culture as possible means of regulation of this reaction. Increased rate of [3H] thymidine incorporation by T lymphocytes were observed when they were cultured with mitomycin-treated autologous non-T cells. This was increased when the stimulating cells were enriched for B lymphocytes and was significantly decreased when the stimulating cells were enriched for monocytes. Addition of monocyte-enriched cells to B-enriched cells in a 1:1 ratio, significantly suppressed the AMLR (P- less than 0.01). Equivalent numbers of monocytes did not suppress T-cell responses to phytohaemagglutinin (PHA). Monocyte-enriched cells were separated from stimulating B-enriched cells by nucleopore (0.6 mu) or dialysis tubing (0.12 mu) in Marbrook chambers. Soluble products released from monocyte-enriched cells also suppressed the AMLR. The significance of the AMLR in vivo is uncertain but it may be important in immunoregulation, monocytes playing an important role.
分离的T细胞对自体非T单核细胞的反应称为自体混合淋巴细胞反应（AMLR）。反应性T细胞表现出免疫记忆和特异性。在所有检测的30名正常个体中均存在这种反应。由于在没有体内过度淋巴细胞增殖证据的情况下，AMLR必须以某种方式受到控制，我们研究了培养中富集的T细胞、B细胞和单核细胞之间的相互作用，作为调节这种反应的可能手段。当T淋巴细胞与丝裂霉素处理的自体非T细胞一起培养时，观察到[3H]胸腺嘧啶核苷掺入率增加。当刺激细胞富含B淋巴细胞时，这种增加更为明显，而当刺激细胞富含单核细胞时，则显著降低。以1:1的比例将富含单核细胞的细胞添加到富含B细胞的细胞中，可显著抑制AMLR（P小于0.01）。等量的单核细胞不会抑制T细胞对植物血凝素（PHA）的反应。在Marbrook小室中，通过核孔（0.6微米）或透析管（0.12微米）将富含单核细胞的细胞与刺激的富含B细胞的细胞分离。富含单核细胞的细胞释放的可溶性产物也抑制AMLR。AMLR在体内的意义尚不确定，但它可能在免疫调节中很重要，单核细胞起着重要作用。
