嗜银核仁组织区计数和增殖细胞核抗原评分是咽癌生存的两个可靠指标。
Argyrophilic nucleolar organizer region counts and proliferating cell nuclear antigen scores are two reliable indicators of survival in pharyngeal carcinoma.
作者信息
Pich A, Chiusa L, Pisani P, Krengli M, Pia F, Navone R
机构信息
Department of Biomedical Sciences and Human Oncology, University of Turin, Italy.
出版信息
J Cancer Res Clin Oncol. 1992;119(2):106-10. doi: 10.1007/BF01209665.
The proliferative activity of pharyngeal carcinoma has been investigated by means of monoclonal antibody PC10 against proliferating cell nuclear antigen (PCNA/cyclin) and argyrophilic nucleolar organizer region (AgNOR) analysis in formalin-fixed, paraffin-embedded biopsies from 45 primary squamous and undifferentiated carcinomas, prior to therapy. The correlation between AgNOR counts and PCNA(PC10) scores was highly significant (r = 0.73; P < 0.0001) as determined by Pearson's correlation coefficient. Moreover, the univariate Kaplan-Meier survival analysis showed a significant correlation between 3- and 5-year survival rates and the mean AgNOR number per tumour cell (P = 0.0003) or the percentage of PCNA(PC10)-positive cells (P = 0.0001). Our results indicate that both AgNOR counts and PCNA(PC10) scores are reliable markers of the proliferative activity of pharyngeal carcinoma in small, routinely processed biopsies, in which they can allow simultaneous evaluation of the histology and tumour cell kinetics.
在治疗前，通过针对增殖细胞核抗原（PCNA/细胞周期蛋白）的单克隆抗体PC10和银染核仁组成区（AgNOR）分析，对45例原发性鳞状和未分化癌的福尔马林固定、石蜡包埋活检组织进行研究，以探讨咽癌的增殖活性。通过Pearson相关系数确定，AgNOR计数与PCNA（PC10）评分之间的相关性非常显著（r = 0.73；P < 0.0001）。此外，单变量Kaplan-Meier生存分析显示，3年和5年生存率与每个肿瘤细胞的平均AgNOR数量（P = 0.0003）或PCNA（PC10）阳性细胞百分比（P = 0.0001）之间存在显著相关性。我们的结果表明，在小的、常规处理的活检组织中，AgNOR计数和PCNA（PC10）评分都是咽癌增殖活性的可靠标志物，它们可以同时评估组织学和肿瘤细胞动力学。
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