Ding S F, Jalleh R P, Wood C B, Bowles L, Delhanty J D, Dooley J, Habib N A
University Department of Surgery, Royal Free Hospital School of Medicine, London.
Gut. 1992 Oct;33(10):1433-5. doi: 10.1136/gut.33.10.1433.
DNA restriction fragment length polymorphism analysis was carried out on a primary and recurrent hepatocellular carcinoma in a hepatitis B virus negative patient. For the primary tumour, allele losses were found on the short arm of chromosome 17 (probe: p144-D6, 17p13) and the long arm of chromosome 5 with the probe Lambda MS8 (5q35-qter); other probes showed either no allele loss or a non-informative pattern. The recurrent cancer also showed allele loss with p144-D6, but not with Lambda MS8. In addition, the recurrent tumour had allele losses with Lambda MS43 (12q24.3-qter), pYNZ22 (17p13), and DNA rearrangement revealed by the probe Lambda MS32 (1q42-43), a pattern not seen in the primary lesion. These results indicate that the second hepatocellular carcinoma was of independent clonality and probably represents a de novo neoplasm rather than a recurrence.
对一名乙肝病毒阴性患者的原发性和复发性肝细胞癌进行了DNA限制性片段长度多态性分析。对于原发性肿瘤，在17号染色体短臂（探针：p144-D6，17p13）和5号染色体长臂（探针Lambda MS8，5q35-qter）上发现了等位基因缺失；其他探针未显示等位基因缺失或呈现非信息性模式。复发性癌症也显示出p144-D6导致的等位基因缺失，但未显示Lambda MS8导致的等位基因缺失。此外，复发性肿瘤存在Lambda MS43（12q24.3-qter）、pYNZ22（17p13）导致的等位基因缺失，并且探针Lambda MS32（1q42-43）显示出DNA重排，这种模式在原发性病变中未见。这些结果表明，第二个肝细胞癌具有独立的克隆性，可能代表一种新发肿瘤而非复发肿瘤。
