Hanson B A
Infect Immun. 1983 Oct;42(1):341-9. doi: 10.1128/iai.42.1.341-349.1983.
Hyperimmune antirickettsial serum was shown to prevent the attachment/penetration stage of Rickettsia tsutsugamushi infection of suspended chicken cells. The extent of the inhibition depended on the serum concentration but not on the presence of complement. The neutralizing activity was reduced by prior adsorption of immune serum with staphylococcal protein A or with intact rickettsiae but was not affected by adsorption with target cells. In the neutralization tests, there was no cross-reactivity between the Karp and Gilliam strains of R. tsutsugamushi. Incubation of rickettsiae with immune serum did not alter their capacity to metabolize glutamate nor grossly damage the permeability barrier function of their cytoplasmic membranes. Although the assay method had the capacity to detect some aggregated infectious organisms, none were found in immune serum-treated suspensions. It was concluded that immune serum may inhibit rickettsial infection by blocking a surface component(s) whose function is necessary for attachment to and/or penetration of target cells.
超免疫抗立克次体血清可防止悬浮鸡细胞被恙虫病立克次体感染的附着/穿透阶段。抑制程度取决于血清浓度，而不取决于补体的存在。用葡萄球菌蛋白A或完整立克次体预先吸附免疫血清可降低中和活性，但用靶细胞吸附则不影响中和活性。在中和试验中，恙虫病立克次体的卡尔普株和吉列姆株之间没有交叉反应。立克次体与免疫血清孵育不会改变其代谢谷氨酸的能力，也不会严重损害其细胞质膜的通透性屏障功能。尽管该检测方法有能力检测到一些聚集的感染性生物体，但在免疫血清处理的悬浮液中未发现。得出的结论是，免疫血清可能通过阻断一种表面成分来抑制立克次体感染，该表面成分的功能对于附着和/或穿透靶细胞是必需的。
