人及大鼠胰岛A细胞中肝细胞生长因子蛋白的免疫组化定位
Immunohistochemical localization of hepatocyte growth factor protein in pancreas islet A-cells of man and rats.
作者信息
Tsuda H, Iwase T, Matsumoto K, Ito M, Hirono I, Nishida Y, Yamamoto M, Tatematsu M, Matsumoto K, Nakamura T
机构信息
Second Department of Pathology, Fujita Health University School of Medicine, Aichi.
出版信息
Jpn J Cancer Res. 1992 Dec;83(12):1262-6. doi: 10.1111/j.1349-7006.1992.tb02756.x.
Hepatocyte growth factor (HGF), a potent mitogen for adult rat hepatocytes in primary culture, has previously been shown to be primarily expressed in the nonparenchymal cells of the liver. Using polyclonal antisera against human and rat HGFs we studied the tissue distribution of HGF immunohistochemically and found the most intense staining in the pancreas islet cells in both man (autopsy cases) and the rat. Differential localization of 4 pancreas islet hormones, glucagon, insulin, somatostatin and pancreatic polypeptide, revealed HGF to be preferentially expressed within the glucagon-positive cells. The results indicate that HGF is primarily produced or stored in A-cells and may act as a growth factor in a paracrine and an endocrine fashion, like various other hormones.
肝细胞生长因子（HGF）是原代培养的成年大鼠肝细胞的一种强效促有丝分裂原，先前已表明其主要在肝脏的非实质细胞中表达。我们使用针对人和大鼠HGF的多克隆抗血清，通过免疫组织化学方法研究了HGF的组织分布，发现在人（尸检病例）和大鼠的胰岛细胞中染色最为强烈。对4种胰岛激素（胰高血糖素、胰岛素、生长抑素和胰多肽）的差异定位显示，HGF优先在胰高血糖素阳性细胞中表达。结果表明，HGF主要在A细胞中产生或储存，并且可能像其他各种激素一样，以旁分泌和内分泌方式作为一种生长因子发挥作用。
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