嗜甲基甲烷八叠球菌MSTA-1对乙酸发酵的动力学研究。
Kinetic studies of acetate fermentation by Methanosarcina sp. MSTA-1.
作者信息
Clarens M, Moletta R
机构信息
Institut National de la Recherche Agronomique, Laboratoire de Biotechnologie de l'Environnement des Industries Agroalimentaires, Narbonne, France.
出版信息
Appl Microbiol Biotechnol. 1990 May;33(2):239-44. doi: 10.1007/BF00176532.
The effect of three parameters (initial acetate concentration, temperature and pH) on the acetoclastic reaction was studied with the thermophilic methanogenic bacterium Methanosarcina sp. MSTA-1. The optimum temperature for growth ranged around 55 degrees C, and optimum pH was 6.5-7.5, giving a minimum generation time of 12.6-13.9 h (mu max = 0.050-0.055 h-1) and a maximum value of the specific acetate consumption rate (qmaxs) of 14-20 mmol/g cells per hour. Contrary to the methane yield, the growth yield was found to be dependent on culture conditions, especially on incubation temperature. Methanosarcina sp. MSTA-1 showed a low affinity for acetate substrate. Growth at 55 degrees C and at constant pH 7 resulted in a Km value and a threshold acetate concentration of 10.7 mM and 0.7 mM, respectively.
利用嗜热产甲烷菌Methanosarcina sp. MSTA-1研究了三个参数（初始乙酸盐浓度、温度和pH值）对乙酸分解反应的影响。生长的最适温度在55℃左右，最适pH值为6.5 - 7.5，最小世代时间为12.6 - 13.9小时（μmax = 0.050 - 0.055 h-1），每小时每克细胞的乙酸盐比消耗速率（qmaxs）最大值为14 - 20 mmol/g。与甲烷产量相反，发现生长产量取决于培养条件，尤其是培养温度。Methanosarcina sp. MSTA-1对乙酸盐底物的亲和力较低。在55℃和恒定pH值7下生长，Km值和乙酸盐阈值浓度分别为10.7 mM和0.7 mM。
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