用于检测鼠伤寒沙门氏菌的压电免疫传感器的研制。

Development of a piezoelectric immunosensor for the detection of Salmonella typhimurium.

作者信息

Prusak-Sochaczewski E, Luong J H, Guilbault G G

机构信息

National Research Council of Canada, Biotechnology Research Institute, Montreal, Quebec.

出版信息

Enzyme Microb Technol. 1990 Mar;12(3):173-7. doi: 10.1016/0141-0229(90)90034-n.

DOI:10.1016/0141-0229(90)90034-n

PMID:1366514

Abstract

A piezoelectric biosensor has been developed for the detection of Salmonella typhimurium. The antibody to Salmonella was immobilized on the crystal by various immobilization procedures. The best result was obtained when antibody was immobilized on the crystal precoated with a thin layer of polyethyleneimine. The response of the coated crystal for S. typhimurium in a microbial suspension was in the range of 10(5) to 10(9) cells ml-1. The time required for a complete interaction between the crystal and the cells appeared to depend upon the cell concentration of the analyzed sample. The antibody-bound crystal lost no activity over 4 days at 4 degrees C and it could be reused for 6-8 consecutive assays.

摘要

一种用于检测鼠伤寒沙门氏菌的压电生物传感器已被研发出来。通过各种固定化程序将抗沙门氏菌抗体固定在晶体上。当抗体固定在预先涂有一层聚乙烯亚胺薄层的晶体上时，可获得最佳结果。包被晶体对微生物悬浮液中鼠伤寒沙门氏菌的响应范围为每毫升10⁵至10⁹个细胞。晶体与细胞之间完全相互作用所需的时间似乎取决于被分析样品的细胞浓度。抗体结合的晶体在4℃下4天内活性无损失，并且可以连续重复使用6至8次测定。